Bead-Based Immunoassays
Formats
Consistent Results with Routine Panels
BD™ CBA Kits provide preconfigured panels for ultimate ease of use. These kits enable multiplex analysis of complex biological samples on a flow cytometer. In contrast to BD CBA Flex Sets, the kits are preconfigured by functional areas of biology (eg, Th1/Th2 or inflammatory cytokines) to measure up to seven analytes simultaneously using capture beads that contain unique amounts of a single red dye. The unique spectral properties of this dye enable analysis of samples on flow cytometers that have a single 488-nm laser or on dual-laser (488-nm or 532-nm and 633-nm) flow cytometers. Each kit comes complete with all of the buffers and reagents necessary to analyze 80 samples.
Consistent packages, pre-optimized, and ready to use
Each assay has been stringently developed for ease of use, rapid data analysis, sensitivity, reproducibility, and quality. Each antibody pair used in the kits is evaluated for dynamic range, sensitivity, and parallel titration curves to native biological samples. In addition, the assay diluent and wash buffers in each kit have been formulated to reduce detrimental effects of serum and plasma proteins on assay performance.
FCAP Array Parameters for BD CBA Kits
Instrument | Reporter Parameter | Clustering Parameters |
|---|---|---|
BD FACSVerse flow cytometer | PE | CBA Red |
BD Accuri C6 flow cytometer | FL2 | FL4 |
BD FACSArray™ bioanalyzer | Yellow | Red |
BD FACSCanto™ II flow cytometer | PE | APC |
BD™ LSR II flow cytometer | PE | APC |
BD FACSAria™ II cell sorter | PE | APC |
BD FACSCalibur™ flow cytometer (single laser) | FL2 | FL3 |
BD FACSCalibur™ flow cytometer (dual laser) | FL2 | FL4 |
