PuraMatrix™ Peptide Hydrogel

BD™ PuraMatrix™ Peptide Hydrogel is a synthetic matrix that is used to create defined threedimensional (3D) micro-environments for a variety of cell culture experiments. To achievoptimal cell growth and differentiation, it is necessary to determine the appropriate mixture of this material and bioactive molecules (e.g., growth factors, extracellular matrix (ECM) proteins, and/or other molecules). BD PuraMatrix Peptide Hydrogel consists of standard amino acids (1% w/v) and 99% water. Under physiological conditions, the peptide component self-assembles into a 3D hydrogel that exhibits a nanometer scale fibrous structure (Figure 1). The hydrogel is readily formed in a BD Falcon™ culture dish, plate, or cell culture insert.

BD PuraMatrix Peptide Hydrogel has been shown to promote the differentiation of hepatocyte progenitor cells,¹ rat pheochromocytoma cells (PC12),² hippocampal neurons,³ and endothelial cells.⁴ Studies have also demonstrated that BD PuraMatrix Peptide Hydrogel supports the attachment of a variety of primary (e.g., neuronal, fibroblast, keratinocyte) and transformed (e.g., MG-63, SH-SY5Y, HEK293, NIH3T3) cell types.^5,6 Other potential applications include stem cell proliferation, tumor cell migration and invasion, angiogenesis assays, and in vivo analyses of tissue regeneration.

References

1. Semino, C.E., et al., Functional differentiation of hepatocyte-like spheroid structures from putative liver progenitor cells in threedimensional peptide scaffolds. Differentiation 71:262 (2003).
2. Holmes, T.C., et al., Extensive neurite outgrowth and active synapse formation on self-assembling peptide scaffolds. PNAS USA 97:6728 (2000).
3. Semino, C.E., et al., Entrapment of migrating hippocampal neural cells in 3D peptide nanofiber scaffold. Tissue Engineering 10:643 (2004).
4. Narmoneva, D., et al., Self-assembling short oligopeptides and the promotion of angiogenesis. Biomaterials 26:4837 (2005).
5. Zhang, S., et al., Self-complementary oligopeptide matrices support mammalian cell attachment. Biomaterials 16:1385 (1995).
6. Thonhoff, J.R., et al., Compatibility of human fetal neural stem cells with hydrogel biomaterials in vitro. Brain Research 1187:42 (2008).
7. Lee, H-S., et al., Clonal expansion of adult rat hepatic stem cell lines by suppression of asymmetric cell kinetics (SACK). Biotechnology and Bioengineering 83:760 (2003).

Quality

Technical Specifications:
• 1% solution (w/v) of purified synthetic peptide
• Packaged material exhibits pH = 2-4
• Quality:
– Tested and found negative for bacteria, fungi, and mycoplasma
– Cell viability ≥ 80% based on cytotoxicity analysis of NIH3T3 fibroblasts
– Identity confirmed using Mass Spectrometry
– Demonstration of fiber formation using a self-assembly assay

Features

Purified Synthetic Peptide Composition
Highly defined material that promotes cell attachment

3D Hydrogel Structure
Assembles into fibrous structure with average pore size of 50-200 nm

Easy Handling
Easily mixed with cells and/or bioactive molecules (e.g., growth factors) prior to gelation; injectable for in vivo studies

Transparent Hydrogel
Samples are readily visualized using standard staining methodologies and microscopy

Established Protocols
3D cell encapsulation cultures; surface plating of adherent cells on microporous membrane inserts and microplates; cell recovery for sub-culturing or biochemical analyses; in vivo injection

Usage

• Peptide sequence promotes cell attachment, but does not activate RGD-dependent integrin signaling
• In the presence of salt-containing solution, the peptide component of BD PuraMatrix Peptide Hydrogel self-assembles and forms a transparent 3D hydrogel
• Biocompatible; devoid of animal-derived material and pathogens

Application

Differentiation of Hepatocyte Progenitor Cells¹
Rat hepatocyte progenitor cells (Lig-8⁷) were encapsulated in BD PuraMatrix Peptide Hydrogel and cultured overnight in defined medium at 37°C. Samples were then used for bromodioxyuridine (BrdU) uptake and in situ immunofluorescence analyses.

Product Details

Catalog Number

354250

Brand

BD™

Name

PuraMatrix™ Peptide Hydrogel

Size

5 ml

Regulatory Status

 RUO

List Price

$200.00   

Labware details - Contents and Storage

Quantity Pack:

1 type :

Quantity Case:

1

Properties

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