Abstract: Cell-based assays are an essential component of basic research, drug discovery as well as regenerative medicine. Throughout these areas of research, there is an increasing need to culture cells reproducibly in better defined conditions. BD PureCoat™ surfaces are chemicallydefined, animal-free enhanced cell culture surfaces.

This presentation will focus on applications demonstrating the use of
BD PureCoat surfaces. More specifically, we show that these surfaces provide better post-thaw recovery of cryopreserved cells, and provide enhanced cell attachment and growth of primary, transformed, transfected, and fastidious cell lines when compared to standard TC vessels. Furthermore, we demonstrate that BD PureCoat surfaces continue to provide robust cell attachment in reduced serum and serum-free media conditions. These surfaces also maintain the integrity of cell monolayers and offer superior consistency during vigorous liquid handling procedures used in cell-based assays. BD PureCoat surfaces provide an alternative to biological coatings, without compromising cell attachment and function.

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