BD OptEIA™ Set ELISA Techniques

Compressed (.mov)

1. Getting started (8.5 mb)
2. Capture antibody preparation and coating (8.6 mb)
3. Washing the plate (7.6 mb)
4. Standard preparation and handling (7.3 mb)
5. Blocking the plate (2.7 mb)
6. Standard and sample addition (8.9 mb)
7. Working detector preparation (9.8 mb)
8. TMB substrate preparation and addition (7.4 mb)
9. Stopping the reaction and reading the plate (4.4 mb)
10. Troubleshooting: Low/No Signal (11.6 mb)
11. Troubleshooting: High Background (8.4 mb)
12. Troubleshooting: Well-to-well Precision (6.6 mb)
13. Troubleshooting: Poor Standard Curve (4.6 mb)

Compressed (.avi)

1. Getting started (20.2 mb)
2. Capture antibody preparation and coating (21.3 mb)
3. Washing the plate (18.7 mb)
4. Standard preparation and handling (15.5 mb)
5. Blocking the plate (6.3 mb)
6. Standard and sample addition (22.3 mb)
7. Working detector preparation (24.7 mb)
8. TMB substrate preparation and addition (18.5 mb)
9. Stopping the reaction and reading the plate (10.8 mb)
10. Troubleshooting: Low/No Signal (28.3 mb)
11. Troubleshooting: High Background (20.4 mb)
12. Troubleshooting: Well-to-well Precision (15.9 mb)
13. Troubleshooting: Poor Standard Curve (10.8 mb)

Uncompressed (.mov)

1. Getting started (26.5 mb)
2. Capture antibody preparation and coating (27.7 mb)
3. Washing the plate (32.3 mb)
4. Standard preparation and handling (23.5 mb)
5. Blocking the plate (11.6 mb)
6. Standard and sample addition (28.4 mb)
7. Working detector preparation (44.7 mb)
8. TMB substrate preparation and addition (32.8 mb)
9. Stopping the reaction and reading the plate (18.0 mb)
10. Troubleshooting: Low/No Signal (46.0 mb)
11. Troubleshooting: High Background (37.4 mb)
12. Troubleshooting: Well-to-well Precision (33.0 mb)
13. Troubleshooting: Poor Standard Curve (14.5 mb)

Uncompressed (.avi)

1. Getting started (25.4 mb)
2. Capture antibody preparation and coating (17.6 mb)
3. Washing the plate (17.4 mb)
4. Standard preparation and handling (15.6 mb)
5. Blocking the plate (5.2 mb)
6. Standard and sample addition (19.0 mb)
7. Working detector preparation (47.0 mb)
8. TMB substrate preparation and addition (16.0 mb)
9. Stopping the reaction and reading the plate (11.3 mb)
10. Troubleshooting: Low/No Signal (32.5 mb)
11. Troubleshooting: High Background (21.8 mb)
12. Troubleshooting: Well-to-well Precision (17.3 mb)
13. Troubleshooting: Poor Standard Curve (12.5 mb)

Click here to download the player to view the quicktime movies (.mov)