BD Pathway™ Software
Features
Software for a full range of bioimaging users and applications
The BD Pathway™ software suite is ideal for high-content cellular analysis and accommodates a full range of bioimaging applications. It provides extensive acquisition, image analysis, data management, and query capabilities to suit the needs of all users, from beginners to advanced HCA professionals.
The BD Pathway software suite provides a complete solution, from image acquisition and analysis to data analysis, visualization, and data management. A comprehensive solution for high-content analysis, the suite allows you to run most HCA applications without the need to purchase additional software. The software suite is both powerful and flexible, delivering a variety of analysis tools. It supports standard unencrypted 16-bit TIF and BMP file formats and exports delimited text files, allowing migration of data to third-party software. The BD Pathway™ 855 platform enables the software to run a number of dynamic live-cell applications including calcium flux and other time-lapse imaging experiments.
| Feature | Benefits |
|---|---|
| Assigns customized metadata to the experiments | BD AttoVision™ 1.7 software’s new metadata capability is based on generation of new custom properties that can be assigned to experiment data acquired on BD Pathway high-content bioimagers. |
| Organizes data into virtual folders for high efficiency | Offers advanced tools that enable transfer of data from the BD Pathway computer or offline client computers to any number of virtual folders—automatically generated storehouses—in the central repository. |
| Links any cross-reference to the experiments | Provides cross-reference capability that associates individual properties with external information. This capability allows users to launch applications and add annotations. |
| Delivers query results based on metadata and virtual folder assignments | Offers sophisticated query functionality that can be used to generate simple or complex queries. The queries can be based on assigned virtual folders, custom or default properties, and custom hierarchies. Queries can be saved and reused for quick access to data. |
| Supports 384-well plate maps | Delivers 384-well plate map compatibility for export to BD™ IDE software. |
| Image preprocessing filters for segmentation (rolling ball background correction, morphological and convolution filters) | Enhanced ability to extract or identify cells or cellular regions of interest for improved image analysis. |
| Object splitting using intensity-based watershed algorithm | Faster and more accurate object splitting for small spots or objects. |
| Band segmentation outputs, including erosion of the nuclear region | Added support for plasma membrane translocation and additional subcellular region analysis. Exclusion regions can be created around the perinuclear region for improved signal: noise in other nucleus/cytoplasm translocation assays. |
| Measurement features (morphometric, intensity, and positional) | Expands the ability to analyze and characterize cellular and subcellular regions or phenotypes. |
| Count objects within objects “Sub Object” | Support for new types of analysis such as DNA damage, co-localization, and spot counting. |
| Flow Cytometry Standard (FCS) export | Export image analysis data for use by third-party software programs. |
| Reactivate experiment settings | Easily apply settings from one experiment to another for better consistency and faster experimental setup. |
| Enhanced screen management | New docking capability for dialogs provides a more customizable, user-friendly workspace. Easier and more intuitive way to acquire and view images and data. |
| Screen layout toolbar | Each user can customize and save personal dialog and toolbar layout settings, to meet their experiment or workflow needs. |
| Improved plate map and image navigation | Improved graphics for easier viewing and navigation of the plate map. Link all open images together to zoom out, zoom in, or pan them altogether at the same time. Saves time and effort when reviewing images. |
| System user instructions available through the Help menu | Provides a way to quickly locate specific instructions to perform tasks using the BD Pathway instrument or BD AttoVision software. |
