BD Pathway bioimaging systems
Features
Advanced, Versatile Optical Design
With BD Pathway high-content bioimagers, cellular images are captured in either confocal or widefield mode, providing the best possible images for analysis. To ensure optimal imaging and results, the BD Pathway system uses advanced optical features including autofocus, movable optics, and a confocal spinning disk.
Advanced optical systems in BD Pathway™ instruments provide even illumination at high intensity across the full spectrum, enabling a wide range of imaging options at multiple resolutions. Laser autofocus allows rapid image acquisition—under five minutes per 96-well plate. Different options for autofocus allow the BD Pathway system to switch from laser-based to camera-based or combined autofocus modes for applications requiring greater control over image acquisition. The unique real-time, true-optical spinning disk provides automated switching between widefield and confocal imaging. This capability delivers high-resolution images without background fluorescence often associated with widefield imaging systems. High-precision x,y,z linear-motor positioning allows fast, precise image montage, also known as tiling, without the need for software processing. This capability helps achieve increased cell counts by acquiring multiple adjacent image fields per sample.
Advanced optical systems in BD Pathway™ instruments provide even illumination at high intensity across the full spectrum, enabling a wide range of imaging options at multiple resolutions. Laser autofocus allows rapid image acquisition—under five minutes per 96-well plate. Different options for autofocus allow the BD Pathway system to switch from laser-based to camera-based or combined autofocus modes for applications requiring greater control over image acquisition. The unique real-time, true-optical spinning disk provides automated switching between widefield and confocal imaging. This capability delivers high-resolution images without background fluorescence often associated with widefield imaging systems. High-precision x,y,z linear-motor positioning allows fast, precise image montage, also known as tiling, without the need for software processing. This capability helps achieve increased cell counts by acquiring multiple adjacent image fields per sample.
The system’s unique motionless stage with movable optics ensures sample stability during image acquisition for loosely adherent and suspension cells. Transmitted light provides the ability to capture bright-field images that can be overlaid onto fluorescence images for precise localization of cellular events. Independent excitation, emission, and dichroic filters are flexible and computer controlled to provide high-speed, fully automated imaging of fluorescently labeled samples. This optical capability allows the system to record high-resolution fluorescence images from multiwell plates and slides.
The system’s unique motionless stage with movable optics ensures sample stability during image acquisition for loosely adherent and suspension cells. Transmitted light provides the ability to capture bright-field images that can be overlaid onto fluorescence images for precise localization of cellular events. Independent excitation, emission, and dichroic filters are flexible and computer controlled to provide high-speed, fully automated imaging of fluorescently labeled samples. This optical capability allows the system to record high-resolution fluorescence images from multiwell plates and slides.
Support for widefield and confocal imaging
A selectable spinning disk—the heart of the BD Pathway system—allows researchers to take advantage of both widefield and confocal imaging. The ability to select different objectives delivers better image quality and analysis. The 3-D capabilities provide quality imaging with collapsed Z stack for each field, while retaining the fluorescence information throughout the depth of the specimen, as shown here.
Support for widefield and confocal imaging
A selectable spinning disk—the heart of the BD Pathway system—allows researchers to take advantage of both widefield and confocal imaging. The ability to select different objectives delivers better image quality and analysis. The 3-D capabilities provide quality imaging with collapsed Z stack for each field, while retaining the fluorescence information throughout the depth of the specimen, as shown here.
Tools to count and measure objects within objects
Using the BD Pathway Object Counting Tools feature, researchers can count and measure objects within objects to analyze subcellular regions via segmentation. This figure shows images of cells stained with a whole cell dye infected with Listeria monocytogenes. The L. monocytogenes are stained with BD Difco Listeria O Antiserum, followed by Alexa Fluor® 488 labeled rabbit secondary antibody.
Tools to count and measure objects within objects
Using the BD Pathway Object Counting Tools feature, researchers can count and measure objects within objects to analyze subcellular regions via segmentation. This figure shows images of cells stained with a whole cell dye infected with Listeria monocytogenes. The L. monocytogenes are stained with BD Difco Listeria O Antiserum, followed by Alexa Fluor® 488 labeled rabbit secondary antibody.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Class I (1) laser product.
