BD Pathway bioimaging systems
Features
Software that Simplifies High-Content Analysis
The BD Pathway software suite simplifies high-content cellular analysis and accommodates a full range of bioimaging applications.
Easy setup and acquisition
For routine analysis, predefined applications are set up by researchers and stored in the system for easy access with a single click. The Assay Launch dialog allows users to quickly select a routine application, enter the wells of the plate to be analyzed, and run the assay.
Intuitive wizards for applications
Applications can be added to the Assay Launch dialog for easy access at any time. Researchers can choose applications provided by BD. BD Pathway™ Wizards require only basic knowledge of imaging, allowing researchers to configure the system using clear, easy to understand directions. The user is guided through the dialog, reviews the summary tab, and launches the application.
Capabilities for advanced bioimaging
Advanced users can develop novel applications unique to their experimental needs. An intuitive macro builder allows researchers to choose from hundreds of instrument settings to meet their precise imaging requirements. The user can then experiment with a variety of settings before selecting those to apply. Once defined, these settings can be saved for running the advanced applications across any number of plates for improved results and reproducibility.
Data analysis and visualization tools
The heatmap display shows the measurement parameter on a well-per-well basis, providing an excellent overview of a drug’s dose-response profile. Multiple dose-response curves can be plotted for comparison between different experimental conditions.
Data analysis and visualization tools
The heatmap display shows the measurement parameter on a well-per-well basis, providing an excellent overview of a drug’s dose-response profile. Multiple dose-response curves can be plotted for comparison between different experimental conditions.
The scatter plot shows the comparison between the area of the cell and rate of rise parameters. The bar chart compares the response levels of two data sets.
The scatter plot shows the comparison between the area of the cell and rate of rise parameters. The bar chart compares the response levels of two data sets.
Embryonic stem cells cultured under feeder-free conditions remain pluripotent.
Human embryonic stem cells (H9) were cultured in mTeSR1 maintenance medium (StemCell Technologies) on BD Falcon™ 96-well imaging plates (Cat. No. 353219) that were coated with BD Matrigel™ hESC-qualified matrix (Cat. No. 354277). Cells were fixed with 4% paraformaldehyde, followed by BD Perm/Wash™ buffer (Cat. No. 554723). Multicolor cell staining was performed with the following antibodies: Sox2 Alexa Fluor® 647 (Cat. No. 560302) pseudocolored yellow, Oct3/4 Alexa Fluor® 555 (Cat. No. 560306) pseudocolored red, and SSEA-4 Alexa Fluor® 488 (Cat. No. 560308) pseudocolored green. Cell nuclei were counterstained using Hoechst 33342 pseudocolored blue. Small images show individual antibody staining. The larger panel represents the merged image. The cells were imaged on a BD Pathway™ 435 bioimager using a 10x objective.
Embryonic stem cells cultured under feeder-free conditions remain pluripotent.
Human embryonic stem cells (H9) were cultured in mTeSR1 maintenance medium (StemCell Technologies) on BD Falcon™ 96-well imaging plates (Cat. No. 353219) that were coated with BD Matrigel™ hESC-qualified matrix (Cat. No. 354277). Cells were fixed with 4% paraformaldehyde, followed by BD Perm/Wash™ buffer (Cat. No. 554723). Multicolor cell staining was performed with the following antibodies: Sox2 Alexa Fluor® 647 (Cat. No. 560302) pseudocolored yellow, Oct3/4 Alexa Fluor® 555 (Cat. No. 560306) pseudocolored red, and SSEA-4 Alexa Fluor® 488 (Cat. No. 560308) pseudocolored green. Cell nuclei were counterstained using Hoechst 33342 pseudocolored blue. Small images show individual antibody staining. The larger panel represents the merged image. The cells were imaged on a BD Pathway™ 435 bioimager using a 10x objective.
Class I (1) laser product.
