BD Pathway bioimaging systems

FAQs

What filter sets are standard with the BD Pathway 855 and 435 high-content bioimagers?

The BD Pathway™ 855 filter configuration:

 Excitation AExcitation BExcitation DichroicEpifluorescence “Emission” DichroicEmission
Filter Wheel PositionDescriptionDescriptionDescriptionDescriptionDescription
1D470/40D340X (334)100% mirrorFura/FITC (72100bs, from Chroma 72000 Set)Fura/FITC (HQ540/50m, from Chroma 72000 Set)
2488/10HQ548/20370DCLPFura/TRITC (73100bs from Chroma 73000 Set)Fura/TRITC (73101m, from Chroma 73000 Set)
3S555/28D460/10open400 DCLP435LP
4D340X (334)HQ560/5550%A/10%B**595LP84101 (84101m, from Chroma 84000 Set)
5D380/10ND*S635/2010%A/40%B**84000 (84000bs, from Chroma 84000 Set)515LP
6360/10488/10  570LP
7380/10open  HQ645/75
8D440/10open  open

* ND denotes neutral density
** Neutral density filters; % denotes transmission from lamp A/B
Notes: Filters are denoted by their center wavelength and bandwidth (e.g. 470/40 = center wavelength = 470 nm, bandwidth = 40 nm (i.e. ± 20 nm); see also www.chroma.com for spectral curves and additional filter information

BD Pathway 435 series filters: Semrock filters

Blue channel dyes: Single sets

  • Exciter #1: 377/50ND (includes 1.0 ND)
  • Exciter: #2 377/50 (BD Pathway 435 only)
  • Dichroic FF409
  • Emitter 435LP (Chroma filter)

Green channel dyes: FITC-3540B set

  • Exciter: 482/35
  • Dichroic: FF506
  • Emitter: 536/40

Orange channel dyes: TRITC-A set

  • Exciter: 543/22
  • Dichroic: FF562
  • Emitter: 593/40

Red channel dyes: CY5-4040A set

  • Exciter: 628/40
  • Dichroic: FF660
  • Emitter: 692/40

BD Pathway 435 filter wheel positions

Excitation wheel
1 = 37750ND
2 = 482/35
3 = 543/22
4 = 628/40
5 = 377/50
6 = open
7 = open
8 = open

Dichroic wheel
1 = FF409
2 = FF506
3 = FF562
4 = FF660
5 = open

Emission wheel
1 = 435LP
2 = 536/40
3 = 593/40
4 = 692/40
5 = open
6 = open
7 = open
8 = open