BD LSRFortessa X-20

Optimal resolution of cell populations
Optimal resolution of cell populations
The high sensitivity of the BD LSRFortessa™ X-20 allows for optimal resolution of lower expression antigens and rare cell subsets. In this example, regulatory T cells (Tregs), characterized by low levels of expression of CD127 and high levels of CD25, were easily identified. Note the resolution of different levels of expression of CD25 and CD127.
Characterization of instrument sensitivity using 8-peak beads
Characterization of instrument sensitivity using 8-peak beads
The sensitivity across detectors of a BD LSRFortessa X-20 equipped with blue (50-mW), red (100-mW), and violet (50-mW) lasers is illustrated by the optimal resolution of SPHERO™ Rainbow 8-peak beads. Note that all peaks are easily identified in various detectors from the blue (A, B), red (C, D), and violet (E, F) lasers.
Analysis of human T-cell subsets
Analysis of human T-cell subsets
Data shows a 10-color panel run on a 3-laser BD LSRFortessa X-20 with a blue/red/violet configuration. The panel was used for defining various subsets of regulatory T cells (A, B), memory/effector CD4+ T cells (C, D, E, F), and memory/effector CD8+ T cells (G, H, I, J).
Analysis of human lymphocyte subsets
Analysis of human lymphocyte subsets
Data shows a 10-color panel run on a 3-laser BD LSRFortessa X-20 with a blue/red/violet configuration. The panel was used for defining various lymphocyte T-cell subsets using lysed whole blood (BD FACS™ lysing solution) from a normal donor.
Analysis of human lymphocyte subsets
Analysis of human lymphocyte subsets
Data shows a 10-color panel run on a 3-laser BD LSRFortessa X-20 with a blue/red/violet configuration. The panel was used for defining various lymphocyte non–T-cell subsets using lysed whole blood (BD FACS lysing solution) from a normal donor.



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