BD LSR cell analyzers
Features
Many innovations are incorporated into the BD™ LSR product line. At the heart of the cytometer, for example, the fluidics system features a true fixed-alignment flow cell gel-coupled to the collection optics to maximize detector signal.
Fluidics system
The fluidics system is pressure driven. Hydrodynamic focusing forces sample cells through the gel-coupled cuvette flow cell where they are interrogated. The flow cell is in fixed alignment with the laser and is gel-coupled to the collection optics. This helps to ensure that the laser is precisely focused on the sample stream and that the maximum amount of emitted light can be collected to generate the greatest amount of signal for added sensitivity in multicolor applications. Fixed alignment also minimizes startup time, improves experiment-to-experiment reproducibility, and enables automated daily quality control.
The sheath container (8 L) and waste container (10 L) are outside the cytometer positioned on the floor.
The optional BD FACSFlow™ Supply System fluidics cart increases capacity and ease of use while maintaining a stable fluidics pressure. It includes an automated sheath and waste fluid control system that reduces daily maintenance by incorporating two 20-L containers (Cubitainers®).
Fluidic sensors maintain constant pressure, while a fluidics monitoring system warns when sheath fluid is low or empty. An alarm sounds when the waste container is full.
Cuvette flow cell
Hydrodynamic focusing within the flow cell forces particles through the cuvette in a single-file stream where laser light intercepts the stream at the sample interrogation point. The unique flow cell design permits particles to flow through the cuvette at a low velocity, allowing longer exposure to laser energy. This ensures optimal resolution and sensitivity.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Class I (1) laser product.
Cubitainer is a registered trademark of Hedwin Corporation.
