BD Influx cell sorter
Sample Data
Measurement of macrophage cell line stimulation using GFP
Murine RAW macrophage cells were transfected with a Green Fluorescent Protein (GFP) and stimulated with varying concentrations of the TLR ligand PAM2. Degradation of GFP fluorescence was measured over a 50-minute time period using a BD Influx system equipped with a 200-mW 488-nm laser. The data were binned and averaged to produce the mean fluorescence for each time point. The BD Influx system is able to make many measurements over short time scales to increase the accuracy of time course studies.
Unique properties can be revealed with polarization-sensitive detectors
A. Viewed with polarization-sensitive detectors on the BD Influx 200-mW, 488-nm laser, liths (scales) and lithed cells of the phytoplankton E. huxleyi can be discriminated using perpendicular and parallel FSC.
B. Polystyrene beads serve as a negative control.
Prochlorococcus and polystyrene bead scatter using the Small Particle Option
A. Small cells of the cyanobacteria Prochloroccocus are visible with the BD Influx 200-mW, 488-nm laser with the Small Particle Option.
B. The 100-nm polystyrene beads would not be visible without the SPO.
Spectral Analyzer Option
Spectral differences between Pseudo-nitzschia and Chlorella upon UV excitation.
Analysis of aquatic samples
Aquatic samples contain a large variety of very small particles. Data shows forward scatter (200 mW, 488 nm) vs fluorescence (692/40) in samples taken at different depths from the Sargasso Sea. Distinction of Prochlorococcus and Synechococcus cyanobacterium clusters by flow cytometry illustrate the exceptional sensitivity of the standard BD Influx forward scatter detector.
Discrimination of small particles can be further improved by measuring fluorescence
A. Representative Hoechst staining of Pelagibacter ubique.
B. Chlorophyll vs PE for raw sea water measured with 200-nm, 488-mW excitation. The BD Influx system offers seven lasers and a large range of filter options.
