BD Influx cell sorter
Features
Flexible Sorting
In addition to two-, four-, and six-way sorting, the BD Influx™ system features special sort modes such as plate sorting, which can be optimized for application requirements.
Accudrop technology simplifies drop-delay determination
BD FACS™ Accudrop technology simplifies setup and eliminates manual calculations normally
Accudrop technology provides convenience and precise sort setup.
Accudrop technology assists the researcher to set the optimal drop-delay value. The drop-delay value can be adjusted while viewing BD Accudrop beads, which are illuminated in the center and side sort streams. The researcher can identify the precise drop-delay value without a fluorescence microscope or manual calculations.
Electronics
The BD Influx system uses parallel electronics to reach a throughput rate of 200,000 events per second, independent of the number of parameters. To achieve very sensitive, low noise electronics, the BD Influx system uses analog electronics in the first stage of signal amplification. The output of the analog peak and hold circuitry is digitized using 16-bit ADCs and placed on a patented parallel processing digital bus. The bus handles real-time compensation as well as other digitized signals, including area and width of up to 8 additional pulses. To sort cells, researchers can select subpopulations through the creation of up to 32 sort regions. A variety of sort modes, optimized to favor purity, yield, or count accuracy, is standard. A custom mode allows researchers to optimize those settings for a specific application.
If purity is of no concern, then the theoretical sort speed limit is as fast as the throughput rate.
In most sorts, however, purity is a major concern and factors such as droplet formation rate, event rate, and sample quality play a deciding role. When these factors are taken into account, the yield can be calculated by using Poisson’s statistics. The BD Influx system will reach >80% of Poisson’s expected yield with properly prepared samples.
Computerized Cell Deposition Unit
For precise sorting, the mechanics and speed of the XY table in the Computerized Cell Deposition Unit (CCDU) allow sorting into microtiter plates or other custom devices such as slides or petri dishes. Single-cell cloning or sorting for single-cell PCR or single-cell imaging techniques is possible with the standard CCDU.
