BD FACSMicroCount

Intensity view results

Results from BD FACSMicroCount and R2A 5- and 10-day Plate Count

BD FACSMicroCount scatter plot showing counts of Streptococcus equi.

Each dot on the plot represents an S. equi cell counted by the BD FACSMicroCount. The result was 3.92 x 10⁹ counts/mL on the BD FACSMicroCount with a corresponding plate count result of 3.40 x 10⁹ cfu/mL.

Correlation of BD FACSMicroCount counts versus plate counts for Streptococcus equi.

Here the BD FACSMicroCount results directly correlate with results from traditional methods (eg, plate count, color changing unit, hemacytometer). Time to result was less than 5 minutes per sample.

Treg sorting, 4-color analysis

Whole human blood was stained in a four-color assay for the CD4+ regulatory T cell (Treg) phenotype using the cocktail from the BD Human Regulatory T Cell Sorting Kit (CD4 PerCP-Cy™5.5, CD25 PE, and CD127 Alexa Fluor® 647) (Cat. No. 560753). CD45RA was not included. Anti-CD3 FITC (clone UCHT1) was added to gate the T cells. Samples were acquired on a BD Accuri® flow cytometer. Data was transformed using prior derived FSC-A and SSC-A parameters, then compensated against single-color controls using FlowJo™ Mac® version 9.1. The CD3⁺CD4⁺ T cells were gated in Boolean fashion, then interrogated for CD25⁺CD127^low phenotype.