BD Accuri C6

Intuitive Software—Master in Minutes

BD Accuri C6 software has an intuitive user interface that was developed based on hundreds of hours observing researchers using flow cytometers.

As a result, most novice flow cytometry users find it so easy to use, that they can collect and analyze data in less than an hour. Software options and instrument controls are clearly visible from the software’s tabbed interface which enables access to the collection, analysis, and statistics functions.


Data is acquired from the Collect tab. Users can create new plots, or copy and re-use plots from this tab. The software supports a full range of selection regions including rectangular, polygon, quadrant, horizontal, and vertical markers.

The Analyze tab displays plots and samples in any combination. In the Analyze tab, users can create color histogram overlays, print multiple plots, and compare samples. Use the Zoom tool to magnify areas of data, instead of voltage adjustments to set the channel range viewed, to better visualize results.

Sample data can be customized in the Statistics tab. Data is displayed in a master table, and statistics can be easily copied and pasted into spreadsheets to facilitate reporting. To simplify creating presentations, plots can be imported into Microsoft® Office applications using drag and drop. BD Accuri C6 software supports live gating, event coloring, export of publication-quality, vector-scalable graphics, and batch analysis, for review or modification of multiple samples for the automatic creation of Microsoft PowerPoint® and Excel® files.

BD Accuri C6 software files can be exported in FCS 3.0 format for seamless importing of user data into flow cytometry analysis programs including FCS Express™ and FlowJo™.

Data collection

The Data Collection function lets users create new plots, hide or delete plots, or copy and re-use plots.

Data analysis

The Data Analysis function lets users create color overlay histograms, print multiple plots, compare samples, and create median statistics.

Statistics

The Statistics function lets users view information in a master table and easily copy and paste into spreadsheets.

Batch analysis
Batch Analysis

 
Sample figure creation using BD Accuri C6 software
Sample figure creation using BD Accuri C6 software

Embryonal carcinoma cell line 2102Ep was stained with antibodies corresponding to the proteins indicated on the histograms. Data was collected on a BD Accuri C6 cytometer. Data shows overlays of single-parameter plots after fluorescence compensation has been applied. Labeling on plots was added in presentation software after plots were dragged and dropped in from the BD Accuri C6 software file.

Stem cell marker comparisons facilitated with overlay plots
Stem cell marker comparisons facilitated with overlay plots

The human embryonal carcinoma (EC) cell line 2102Ep can serve as a reference standard for human embryonic and induced pluripotent stem cells because it expresses most of the same markers. After staining with antibodies to stem cell markers, 2102Ep EC cells were collected and analyzed on a BD Accuri C6. Overlays of single-parameter histograms were drawn with BD Accuri C6 software. Plots were dragged and dropped into presentation software, where labels were added. Note: The curve for SSEA-1 (gray in plot A), a negative pluripotency marker, is almost identical to the negative control (black).



Microsoft, PowerPoint, and Excel are registered trademarks of Microsoft Corporation.

FlowJo is a trademark of Tree Star, Inc.

FCS Express is a trademark of De Novo Software.