BD Accuri C6

Sample Flexibility with Optional Walkaway Sample Loading

A unique low-pressure pumping system drives the fluidics. A sheath-focused core enables event rates of up to 10,000 events per second and a sample concentration of over 5 x 106 cells per mL. In addition, the system derives sample volume and can calculate absolute counts or sample concentration per microliter.

The non-pressurized system supports any brand of 12 x 75-mm (or smaller) sample tubes, including microcentrifuge tubes and tubes made of polypropylene or polystyrene. The BD Accuri C6 cytometer simplifies system maintenance with automatic cleaning cycles on instrument shutdown. The system can employ laboratory-grade water for sheath fluid, reducing operating costs.

For walkaway convenience, the optional BD CSampler™ accessory offers reliable and easy-to-use automation. The system supports 48- and 96-well plates and deep-well plates, and is also supplied with a 24-tube rack for standard 12 x 75-mm tubes. They are processed directly in the BD Accuri C6 cytometer, saving time. The BD CSampler adds minimal footprint to the BD Accuri C6, about three feet square for the pair, keeping the benchtop free for other uses.

To streamline sample processing, the BD CSampler allows multiple collection settings to be applied to plate or tube runs. To process a sample immediately, a run can be paused using the Interrupt function. When the priority operation is complete, the original plate can be returned to the BD CSampler to resume the original run. Easy-to-read software messages keep users informed of system status.

BD Accuri C6 with CSampler option

Applications in kinetic analysis of cellular responses

Changes to intracellular calcium (Ca2+) levels can occur rapidly, in some cases within nanoseconds of stimulation, and obtaining accurate data is a significant research challenge. To add test compounds to the cell suspension, a “stop-flow” method is often used in which sampling is paused, the sample tube opened, the agonist added, and the tube resealed. This technique leaves a gap in data collection that may miss essential changes in Ca2+ levels.

The BD Accuri C6 employs non-pressurized peristaltic pumps in an open fluidics system. Open tubes allow convenient addition of test compounds to the cell suspension without interrupting sampling. This “continuous-flow” method enables non-stop analysis of calcium flux and other kinetic cellular responses, such as pH, reactive oxygen and nitrogen species, mitochondrial membrane potential, and nanoparticle uptake.

Gap-free analysis of intracellular calcium

Gap-free analysis of intrcellular calcium

A The three upper cytograms show calcium data obtained on a Beckman Coulter CyAn™ ADP using the "stop-flow" method, showing time gaps when control and test compounds were added.

B The three lower cytograms show data obtained on a BD Accuri C6, adding the same compounds in open Eppendorf® tubes without interrupting sample acquisition. No time gaps were observed; otherwise, both methods obtained comparable data.

Data from Vines A, McBean GJ, Blanco-Fernández A. A flow cytometric method for continuous measurement of intracellular Ca2+ concentration. Cytometry Part A. 2010;77:1091-1097. Reproduced courtesy of the authors.

CyAn is a trademark of Beckman Coulter, Inc.

Eppendorf is a registered trademark of Eppendorf AG.