T Cell

A Choice of Techniques for Quantification of Secreted Cytokines

Secreted cytokines in samples can be quantified by various techniques. BD™ Cytometric Bead Array (CBA) can simultaneously quantify multiple cytokines from the same sample, while ELISA is a useful assay for measuring levels of single cytokines. BD™ ELISPOT reagents determine the frequency of cytokine-producing cells, while BD™ In Vivo Capture Assays allow sensitive and direct measurement of cytokine production in vivo (in mice).

BD Cytometric Bead Array (CBA)

BD CBA is a bead-based immunoassay for quantifying multiple proteins simultaneously using flow cytometry. The BD CBA portfolio includes assays for the measurement of a variety of soluble and intracellular proteins including cytokines, chemokines, growth factors, and phosphorylated cell signaling proteins. This technology makes BD CBA especially suitable for studying events involved in T-cell differentiation.

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BD ELISA reagents

ELISA is a useful assay for quantitating single cytokines. BD offers maximum flexibility, offering a large menu of pre-optimized, ready-to-use BD OptEIA™ kits and sets, as well as individual matched capture and detection antibody pairs with corresponding recombinant protein standards.

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BD ELISPOT technology

The BD ELISPOT assay is a powerful tool for detecting and analyzing individual cells that secrete a particular protein in vitro. This technology enables determination of the frequency of cytokine-producing cells in a sample. It is highly sensitive and allows for downstream analysis.

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BD in vivo capture assays

The BD In Vivo Capture Assay is a complementary technology to ELISA and CBA. It is used for sensitive and direct measurement of cytokine production in vivo (in mice) through the injection of a cytokine-specific antibody and subsequent analysis of collected serum in an ELISA-type assay. The in vivo capture assays are easy to use and yield highly sensitive and directly correlated results.

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Comparison of technologies for measuring cytokines

Allows detection of multiple cytokines in same experiment X   X
Can obtain phenotype of specific cells expressing cytokine of interest     X
Can measure quantity of cytokine secreted X X