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The FACSCalibur System


The FACSCalibur System
The Automated, Four-Color Flow Cytometry System

FACSCalibur Technical Specifications

FACSCalibur is another BD first: the only four-color, dual-laser benchtop flow cytometry system available today capable of both analyzing and sorting. Combined with its automated sample loader, a wide variety of reagents, and powerful software, FACSCalibur offers the high throughput necessary to meet productivity requirements without compromising the flexibility and performance essential for today’s busy, cost-conscious laboratory.

Compact and easy to use, FACSCalibur can quickly perform routine tasks including immunophenotyping, reticulocyte enumeration, DNA analysis, and absolute counting. And with its superior four-color analysis and sorting capabilities, FACSCalibur is a highly sensitive tool, enabling laboratories to perform sophisticated cellular work in areas such as genetics, multicolor analysis, and immune function (for example, using FastImmune).

FACStation Data Management System

The ease of use of the FACSCalibur flow cytometer is enhanced by its user-friendly data management system. FACStation takes advantage of the intuitive Macintosh environment. It performs all the computing tasks required for fast, accurate results, including instrument setup, data collection, analysis, and management. FACStation’s automated software and high-performance acquisition and analysis tools make plotting, gating, statistical analysis, and reporting easier.

FACS Loader: Walkaway Automation Enhances Productivity

With the FACS Loader option, FACSCalibur affords walkaway automation for even the most demanding applications. Featuring removable 40-tube carousels, on-board mixing, and our WorklistManager software, FACS Loader offers unsurpassed flexibility.

Dual-Laser, Four-Color Flow Cytometry

FACSCalibur offers the industry standard in four-color analysis capability: two lasers spatially separated for high sensitivity, minimal compensation, and maximum flexibility in fluorochrome selection.

Cell Sorting: Innovative Technology Makes Cell Sorting Routine

FACSCalibur is the only benchtop flow cytometry system available today capable of sorting cells of interest.

With FACSCalibur, sorting is routine—no time-consuming setup procedures are required. Just gate the population of interest and click ACQUIRE—FACSCalibur does the rest. Sorting occurs in a completely enclosed, aerosol-free environment for enhanced safety when processing biohazardous samples. Multiparameter flow cytometry, combined with cell sorting, adds an additional dimension to the information you gain from your sample. Purification of subpopulations through sorting eliminates contaminants that can interfere with sensitive assays, increasing the number of target cells in the sample, or confirming flow cytometric findings. Cell sorting is ideal for verifying cell morphology and performing subsequent molecular techniques such as FISH, PCR, or functional assays. Cross-correlation of flow cytometric data with post-sort results is a powerful tool in cellular analysis.

Cells sorted by the FACSCalibur are collected into test tubes, or if desired, cells can be sorted directly onto filters or cell culture inserts by the Cell Concentrator Module option. The Cell Concentrator Module recovers sorted cells conveniently, without centrifugation.

Service and Support When It Counts

Because we understand how critical it is to have your system up and running, Becton Dickinson backs FACSCalibur, its software and reagents, with FACService, the most comprehensive and highly rated service and support program in the industry. FACService ensures maximum instrument up-time and optimal performance with guaranteed response times, preventive maintenance programs, and highly trained service and application specialists.

And now BD is first in the industry to go on-line with FACSConnect* remote diagnostics system. Through the use of a modem and Timbuktu software, FACSConnect allows our support staff to perform real-time assessment and support of your flow cytometer by monitoring on our computer screen exactly what you see on yours. This efficient troubleshooting process enhances productivity with fewer workflow interruptions, and it’s a direct training opportunity for lab personnel.

The FACSCalibur System

With its innovative reagents, powerful application software, and advanced cytometry hardware, FACSCalibur is the answer to your laboratory needs. Its built-in versatility meets the current demands of both clinical and research environments. Its modularity takes you into the future. Best of all, the FACSCalibur system is backed by the leader in flow cytometry, BD.

For more information, contact your local BD Biosciences representative.

* Not available in all locations.

23-3090-01W

An indicates this product is for in vitro diagnostic use. All other products listed are for research use only; they are not for use in diagnostic or therapeutic procedures.