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Brochures
FACSCalibur Technical Specifications
| Performance |
| Fluorescence Sensitivity |
Estimated detection limit is 750 molecules of equivalent soluble Fluorescein |
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| Fluorescence Resolution |
Coefficient of variation in FL2-Area of < 3%, full peak for propidium iodide–stained chicken erythrocyte nuclei |
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Forward and Side
Scatter Sensitivity |
Sensitivity enables the separation of fixed platelets from noise |
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Forward and Side
Scatter Resolution |
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes |
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| Excitation Optics |
| Optical Platform |
Fixed optical assembly |
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| Lasers |
15 mW 488 nm, air-cooled argon-ion laser; life expectancy >5,000 hours
Optional
second red diode laser: nominally 635 nm |
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| Beam Geometry |
Prismatic expander and achromatic spherical lens provide 22 x 66-µm
elliptical beam for argon-ion laser. Nominally 15 x 61-µm elliptical beam for red diode
laser |
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| Emission Optics (Top) |
| Optical Coupling |
Quartz cuvette is coupled to emission lens by refractive index matching
optical gel for optimum collection efficiency |
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| Background Rejection |
Obscuration blade and slit minimize unwanted laser radiation at the
detector |
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| Forward Scatter
Detector and Filter |
High-performance solid-state silicon detector; 488 nm bandpass filter for
clear signal detection and red diode laser signal rejection |
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| Side Scatter Detector |
Photomultiplier using Brewster-angle beam splitter in the emission optical
train |
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| Fluorescence Detectors
and Filters |
High performance, high dynamic range photomultipliers with bandpass
filters: 530 nm (FITC), 585 nm (PE/PI), 661 nm (APC), and >650 nm (PerCP) with base
unit, >670 nm (APC) with FL4 option |
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| Fluidics (Top) |
| General Operation |
Front key panel control provides three modes: RUN, STNDBY, and PRIME;
automatic standby mode conserves sheath fluid by stopping sheath flow when no sample tube
is installed |
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| Fluid Reservoirs |
Easily accessible 4L capacity sheath and waste containers are housed in a
convenient pull-out drawer; level detectors automatically indicate low levels of sheath or
high levels of waste |
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| Sample Flow Rates |
Three selectable flow rates of 60 µL/min, 35 µL/min, and
12 µL/min. Pressure difference between sheath and sample is regulated and monitored;
particle velocity in flow cell is approximately 6 meters/sec |
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| Quartz Cuvette |
Internal cross-section is rectangular 430 x 180-µm; external surfaces are
anti-reflection coated for maximum transmission of laser light |
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| Sample Concentration |
Single-cell suspension of 105 to 2 x 107
particles/mL recommended range |
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| Signal Processing (Top) |
| Workstation Resolution |
1024 channels on all parameters |
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| Dynamic Range |
Logarithmic amplifiers for SSC, FL1, FL2, FL3, and FL4 (with FL4 option)
provide four log decade range |
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| Fluorescence
Compensation Networks |
Fluorescence spectral overlap can be compensated between FL1 and FL2,
between FL2 and FL3 channels, and between FL3 and FL4 (with FL4 option) |
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| Pulse Processing |
Width and Area measurements for discriminating doublets; available for all
fluorescence parameters |
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| Time |
Time available correlated to any parameter for kinetic experiments or
other applications. |
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| Scatter Optics (Top) |
| Forward Scatter Range |
Obscuration blade and optics aperture limit the forward scatter radiation
collection to a half-cone angle range of ~0.7 to 10 degrees |
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| Background Reduction |
Obscuration blade and 100-µ slit minimize unwanted laser radiation at the
detector. 488 nm bandpass filter in front of FSC and SSC detectors |
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| Forward Scatter
Detector |
Solid state silicon detector with spectral response from 300 nm to 1100 nm |
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| Side Scatter Detector |
High-performance photomultiplier using Brewster angle beam splitter in the
emission optical train |
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| Sample Loading
Specifications (Top) |
| Sample Delivery |
Tube-lifter design with multiple sensors which verify rack identification
and tube position |
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| Rack Capacity |
40 (12 x 75-mm) tubes per rack |
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| Rack Support |
Up to 16 racks per FACS Loader |
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| Data Entry |
Sample information, reagent panels, and rack information can be defined
for up to 640 tubes (40 tubes x 16 racks) at a time |
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| Loader Control |
Automated control through WorklistManager software and manual control with
stat interrupt capability through FACS Loader electronic keypad |
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| Barcode Scanner (optional) |
Automates data entry for Codabar, Code 39, Interleaved 2 of 5, Code 2 of
5, and Code 128 |
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| Mixing Mode |
Adjustable High Energy and Low Energy mix |
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| Data Management System
(Top) |
| Workstation |
FACStation |
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| Central Processing
Unit (CPU) |
Two Intel Xeon Quad-Core Processing |
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| Memory |
1GB RAM |
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| Level 2 Cache |
4MB per processor |
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| Data Storage |
250GB 7200-rpm Serial ATA 3 Gb/s |
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| Networking |
On-board Ethernet, built-in AppleTalk Networking, and File Sharing |
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| CD ROM |
16x SuperDrive |
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| Monitor |
17-inch MultiSync Monitor |
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| Data File Structure |
Flow Cytometry Standard (FCS) 2.0 ASCII results file for data export |
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| Sorting Specifications
(Top) |
| Sorting Purity |
> 95% |
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| Capture Rate |
300 cells/sec |
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| Sort Modes |
Three modes (all aerosol-free): single cell, exclusion, and recovery |
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| Recovery |
Depends upon sample and sorting conditions, > 50% |
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| Sterile Sorting |
System design allows for aerosol-free sterile sorting |
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| Remote Diagnostics
(Top) |
| Remote Diagnostics */
Modem * Not available in all locations |
Provided for direct customer support instrument interaction |
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| Operating Environment (Top) |
| Temperature |
16°
to 29°C
(60°-85°F) |
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| Humidity |
10% to 90% relative, non-condensing |
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| Air Filtering |
Excessive dust and smoke must be avoided |
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| Lighting |
Optics and detectors are shielded from room lighting |
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| Size |
Sensor module: width - 91.4 cm (36 in); depth - 61.5 cm (24.2 in); height - 67.3 cm
(26.5 in); 124.5 (49 in) with cover open
Computer: 48 x 41 x 54 cm (19"L x
16"D x 23"H)
Printer: 48 x 41 x 54 cm (19"L x 16"D x 23"H) |
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| Weight |
Sensor Module: 109.1 kg (240 lbs)
Computer: 50 kg (110 lbs) |
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| Technical Assistance (Top) |
BDIS Customer
Support Center
2350 Qume Drive
San Jose, California 95131 - 1807, USA
(800)448-BDIS (2347)Customers outside the
US, contact your local BD
representative or distributor. |
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Specifications subject to change without notice.
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