Webinars
The BD Biosciences Webinar Series is an ongoing, informative program in which senior scientists present online seminars covering a broad range of topics.
Multicolor Flow Cytometry: Setup and Optimization on the BD Accuri™ C6 Flow Cytometer
Date: Wednesday, February 15, 2012
Time: 2:00 PM to 3:00 PM Eastern Time
Speaker: Clare Rogers, MS, Senior Marketing Applications Specialist, BD Biosciences
Abstract
Recent innovations have dramatically improved the ways that flow cytometry can help identify and functionally characterize complex populations, and detect and monitor multiple targets at the same time. Join BD Accuri scientist Clare Rogers for a look at how multicolor flow cytometry can be used on the BD Accuri C6 to analyze multiple cellular parameters simultaneously. During this webinar, a reprise of a popular webinar from last September, we will focus on key principles of multicolor panel design, instrument setup, sample preparation, and assay optimization as they apply to the BD Accuri C6. Using a complex blood immunophenotyping application as an example, we will also highlight online tools such as the new BD FACSelect™ Multicolor Panel Designer.
Protocols and Tricks for Clearance Experiments and Drug Transport Using Hepatocytes: Uptake and Efflux
Date: Thursday, February 23, 2012
Time: 11:00 AM to 12:00 PM Eastern Time
Speaker: Rongjun Zuo, PhD., Staff Scientist / R&D, BD Biosciences
Abstract
Primary human hepatocytes are often considered the “Gold Standard” for drug metabolism studies. However, they are now also gaining popularity in drug clearance involving drug transporters. Specifically, plateable human hepatocytes are a suitable model for assessing in vitro metabolic stability of drugs requiring long term incubation. When cultured in a sandwich format with Collagen I and BD Matrigel™ they form an in vivo-like hepatobiliary network that facilitates the assessment of efflux and uptake drug transporter activities.
This webinar will focus on protocols and tricks for working with plateable primary human hepatocytes in the above applications. Emphasis will not only be placed on the detailed procedures for thawing/plating cryopreserved human hepatocytes, assay procedures using probe substrates, and data analysis, but also on critical steps for performing a successful assay.
