The BD Biosciences Webinar Series is an ongoing, informative program in which senior scientists, professionals in the community, and clinicians present online seminars covering a broad range of topics.

Flow Cytometry Applications in Cell Biology and Cancer Research

Date: Wednesday, March 18, 2015
Time: 12 PM EDT / 9 AM PDT / 5 PM CET
Daniela Trisciuoglio, PhD, Researcher
Regina Elena National Cancer Institute, Rome, Italy

Nil Emre, PhD, Manager, Research & Development
BD Biosciences, San Diego, CA, USA

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Flow cytometry is an indispensable methodology for cancer research because it supports the multiparametric analysis of individual cells and subpopulations in heterogeneous samples. This presentation will showcase how the BD Accuri™ C6 flow cytometer system fits into the labs of cancer researchers and cell biologists.

Dr. Emre will discuss flow cytometric applications for the detection of apoptosis, viability, cell cycle, proliferation, and DNA damage. She will also cover cell surface signatures, cell surface marker screening using BD Lyoplate™ panels, and dose-response curves. Dr. Trisciuoglio will describe how she uses these assays to study the thiazole derivative CPTH6 as a potential antineoplastic drug in preclinical models of hematological and solid tumors, including data obtained on the BD Accuri C6.

Dr. Trisciuoglio has been actively involved in preclinical research since receiving her degree in biology in 1999. Since 2000, she has been a junior researcher at the Elena Cancer Institute (IRE) of Rome, an IRCCS that represents a national and international referee center for clinicians and researchers in the field of oncology. In 2003 she was a recipient of a 3-year fellowship from the Italian Foundation for Cancer (AIRC) working on a project aimed at defining the role of bcl-2 anti-apoptotic protein in tumor progression and angiogenesis, under the supervision of Dr. Donatella Del Bufalo. In particular, her research demonstrated that modulation of bcl-2 in human cancer cells consistently regulates in vitro cell invasion and in vivo tumor growth, and synergizes with hypoxia to increase angiogenesis.

After her residency at the National Cancer Institute in Frederick, Maryland thanks to a 1-year AIRC abroad fellowship, in 2008 she started a PhD program in physiology and molecular toxicology at the University of Siena, under the supervision of Dr. Donatella Del Bufalo. She has gained experience in apoptosis and cell cycle regulation, neo-angiogenesis, advanced flow cytometry, and design and analysis, including statistical analysis, of preclinical experiments.

In 2012, she was funded by AIRC with a "My First AIRC Grant," a 3-year program that supports junior scientists. This grant allowed Dr. Trisciuoglio to establish her independent research. In this context, she is evaluating the antitumoral properties of small molecules inhibiting histone acetyltransferse or histone deacetylase activity in cancer cell lines of different histological origin, including cancer stem cell-derived models of lung carcinoma.

Dr. Emre received her PhD in Biological Chemistry from the University of California at Los Angeles. She pursued post-doctoral research at The Scripps Research Institute, focusing on high-throughput screening approaches to identify small molecules that regulate human embryonic stem cell differentiation. Dr. Emre joined BD Biosciences in 2008, where her group develops and supports antibody-based research reagents and applications for cell biology research.

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