Label your cells when performing cell migration, cell invasion, and endothelial cell tubulogenesis studies.
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MDA-MB-231 breast adenocarcinoma cells stained with BD™ Calcein AM Fluorescent Dye. |
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NG-108 neuroblastoma cells stained with BD™ DiIC12(3) Fluorescent Dye. |
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Fluorescent dyes are commonly used for the detection of live cells and key functional activities in a variety of cell-based assays. The fluorescent dyes, calcein acetoxymethylester (calcein AM) and DiIC12(3), can be used with BD Falcon™ FluoroBlok™ Cell Culture Inserts and Insert Systems to label cells when performing analyses such as tumor cell invasion, endothelial cell migration, endothelial cell tubulogenesis, and other cell-based assays.
The cell viability indicator calcein AM, is a non-fluorescent, cell permeant compound that is hydrolyzed by intracellular esterases into the fluorescent anion calcein. BD™ Calcein AM Fluorescent Dye can be used to fluorescently pre- and post-label viable cells to perform kinetic and endpoint experiments, respectively. DiIC12(3) is a lipophilic neuronal tracer that is commonly used for the labeling of neuronal projections as well as lipid bilayers in other cell types. Since DiIC12(3) exhibits low toxicity and minimal effects on cell viability, this dye can be used to pre-label cells for a variety of applications. BD™ DiIC12(3) Fluorescent Dye can be used to fluorescently label viable cells for assays such as tumor cell invasion or endothelial cell migration. Moreover, cells are known to tolerate different fluorescent dyes for varying amounts of time in culture. While cells labeled with calcein AM should be exposed to the dye for less than eight hours, DiIC12(3) can be used for several days in culture without adversely affecting cells.
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- Reliable performance
- Lot-to-lot consistency
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- Tumor cell invasion
- Cell migration
- Chemotaxis
- Cell viability
- Cell attachment
- Cell proliferation
- Multi-drug resistance
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Dye
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Application
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BD™ Calcein AM
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pre- or post-labeling
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494/517
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BD™ DiIC12(3)
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pre-labeling
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549/565
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BD Calcein AM Fluorescent Dye
- purity >95%
- Extinction Coefficient > 6,000 M-1cm-1
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BD DiIC12(3) Fluorescent Dye
- Purity >95%
- Extinction Coefficient > 150,000 M-1cm-1
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(Extinction Coefficients are compound in methanol)
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Product is shipped on dry ice. Upon receipt, store immediately at -20ºC. Stable for at least three months from date of shipment.
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THP-1 chemotaxis (representative data). Cells were pre-labeled with BD Calcein AM Fluorescent Dye. MCP-1, monocyte chemoattractant protein-1.
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HT-1080 migration (lot-to-lot data). Cells were post-labeled with BD Calcein AM Fluorescent Dye.
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HT-1080 cell invasion through BD Matrigel™ Matrix. HT-1080 or 3T3 cells (25,000/well) were pre-labeled with BD™ DilC12(3) Fluorescent Dye. Cell invasion was measured at various time points over 24 hours.
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Description
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Cat. No.
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Qty.
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Cat. No.
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Qty.
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BD Falcon™ FluoroBlok™ Cell Culture Inserts
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| 3.0 µm inserts for 24-well plates |
351151 |
48 |
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| 8.0 µm inserts for 24-well plates |
351152 |
48 |
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BD Falcon™ FluoroBlok™ 24-Multiwell Insert Systems
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| 3.0 µm insert plate with 24-well plate and lid |
351155 |
1 |
351156 |
5 |
| 8.0 µm insert plate with 24-well plate and lid |
351157 |
1 |
351158 |
5 |
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BD Falcon™ FluoroBlok™ 96-Multiwell Insert Systems
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| 3.0 µm insert plate with 96-well plate and lid |
351161 |
1 |
351162 |
5 |
| 8.0 µm insert plate with 96-well plate and lid |
351163 |
1 |
351164 |
5 |
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BD BioCoat™ Angiogenesis Systems
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Endothelial Cell Invasion
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| 3.0 µm insert plate with 24-well plate and lid |
354141 |
1 |
354142 |
5 |
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Endothelial Cell Migration
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| 3.0 µm insert plate with 24-well plate and lid |
354143 |
1 |
354144 |
5 |
| 3.0 µm insert plate with 96-well plate and lid |
354147 |
1 |
354148 |
5 |
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Endothelial Cell Tube Formation
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| 96-well black/clear BD Optilux™ Microplate |
354149 |
1 |
354150 |
5 |
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BD BioCoat™ Tumor Invasion System
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| 8.0 µm insert plate with 24-well plate and lid |
354165 |
1 |
354166 |
5 |
| 8.0 µm insert plate with 96-well plate and lid |
354167 |
1 |
354168 |
5 |
To place an order in the U.S., contact our Customer Service Department at: tel: 877.232.8995; fax: 800.325.9637 or 858.812.8889; e-mail: BDBCustomerService@bd.com
For technical assistance, contact Technical Service at: tel: 877.232.8995 or 978.901.7389; fax: 978.901.7491; e-mail: labware@bd.com
Outside the U.S., contact your local distributor or nearest BD Biosciences office.
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For Research Use Only. Not intended for use in diagnostic or therapeutic procedures.
Fluorescent dyes manufactured for BD by AnaSpec, Inc.
BD, BD Logo, and all other trademarks are the property of Becton, Dickinson and Company. ©2006 BD
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