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BD FACSAria II cell sorter

Fluidics

Sample to Sort Collection

Optics Multicolor Controls and Analysis Services

Aerosol management is
engineered into the system.

After leaving the nozzle, particles pass through the sort block where they are deflected into a collection device in the sort collection chamber. The sort block houses the high-voltage deflection plates used to deflect side streams during sorting. A novel deflection plate design fixes plates in position for more efficient and reproducible deflection.

The aspirator and aspirator drawer are also housed in the sort block. The aspirator drawer keeps the sort collection tubes covered until sorting begins and automatically closes to protect sort collection tubes when the Sweet Spot is on and a clog is detected.

Aerosol management

Engineered with aerosol management in mind, the BD FACSAria II features a sealed pathway from the sample injection chamber to the sort collection tubes. For an added level of aerosol management, the BD™ Aerosol Management Option (AMO) evacuates the sort collection chamber and traps aerosolized particles during sorting.

Sample injection chamber

The sample injection chamber is where the sample is introduced into the flow cytometer. During acquisition, the chamber is pressurized, forcing the sample to the cuvette flow cell. Sample injection chamber temperature control and agitation are standard with the BD FACSAria II cell sorter. The BD FACSDiva software controls both sample agitation and temperature settings.

After a sample tube is run, both the inside and outside of the sample injection tubing are flushed to minimize carryover. A variety of tube holders are provided, from 15-mL centrifuge tubes to 1.0-mL microtubes. New 35- and 50-micron sample line filters minimize clogging during a sort.

Sort collection chamber

The new universal mount in the sort collection chamber makes inserting the tube holders easier. This design is more robust and simplifies maintaining aseptic conditions. Temperature control for both sort collection tubs and plates is available as an option.

Nozzle selection and design

An expanded set of nozzles lets users sort a wide range of particle sizes. Four nozzle sizes are supported: 70, 85, 100, and 130 microns. An improved nozzle insertion design offers tight registration for a secure fit. This means a reproducible drop profile after every insertion, allowing for reproducible instrument setup. Nozzles are readily accessible and easy to change. Software enhancements include a nozzle-specific sort setup feature that matches pressure settings to the nozzle in use.

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