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BD FACSAria II cell sorter

Fluidics Sample to Sort Collection Optics

Multicolor

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Detects up to 15 parameters simultaneously for multicolor flexibility.

For many users, the capability of an advanced cell sorter is defined by its flexibility, which in turn is defined by the number of parameters that can be detected simultaneously. To provide maximum flexibility, the BD FACSAria II cell sorter can be configured with up to four air-cooled, solid state lasers and can detect up to 15 parameters simultaneously—13 fluorescent markers and two scatter parameters when fully equipped.

A standard system is equipped with an octagon containing six PMTs that detect light from the 488-nm (blue) laser, and a trigon containing two PMTs that detect light from the 633-nm (red) laser. The third laser option adds an additional trigon containing two PMTs to detect light from the 405-nm (violet) or 375-nm (Near UV) lasers. Standard instruments can be configured with all four lasers.

375-nm Near UV Laser

The 375-nm Near UV laser option is available to expand the range of application choices. Measuring only 19 × 7 × 7 cm, the fully-housed 375-nm laser fits in the small space immediately left of the flow cell. The laser produces a 10-mW focused beam to illuminate the cells in the flow cell without the aid of fiber optics.

375-nm Near UV laser

The top hat design of the 375-nm Near UV laser modifies the usual Gaussian beam (in red) that illuminates a sample at higher intensity in the middle of the beam (in blue) and lower intensity at the edges. The composite "square" beam illuminates the middle and edges of the sample core stream at the same intensity. The "square" beam almost triples the amount of light at the sample intercept.

Mouse stem cell side population detection

As a consequence of the even sample illumination, cells have a greatly improved coefficient of variation (CV).

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