Frequently Asked Questions: Sample to Sort Collection
What improvements have been made to the deflection plates and why?
How does the aspirator drawer protect the collection tubes?
Are temperature control and agitation standard?
How is tubing flushed to reduce sample carryover?
What sizes of tubes holders are provided with the BD FACSAria II?<
What sizes of sample line filters are supported?<
How can I sterilize the sample line filters?
How was the BD FACSAria II engineered for aerosol management?
Are there additional features for aerosol management?
How does the new universal mount for sort collection tubes help maintain aseptic conditions?
What sizes of nozzles does the BD FACSAria II support?
What other improvements were made to the nozzle design?
What improvements have been made to the deflection plates and why?
New curved deflection plates fix the plates in position for more efficient sample stream deflection.
How does the aspirator drawer protect the collection tubes?
The aspirator drawer keeps the sort collection tubes covered until sorting begins and automatically closes to protect sort collection tubes when the Sweet Spot is on or a clog is detected.
Are temperature control and agitation standard?
Yes. Sample injection chamber temperature control and agitation are standard with the BD FACSAria™ II cell sorter. BD FACSDiva software controls both sample agitation and temperature settings.
How is tubing flushed to reduce sample carryover?
After a sample tube is run, both the inside and outside of the sample injection tubing are flushed to minimize carryover from one sample to another.
What sizes of tubes holders are provided with the BD FACSAria II?
A variety of tube holders are provided to support 1.0-mL microtubes, 12 x 75-mm tubes, and 15-mL centrifuge tubes.
What sizes of sample line filters are supported?
The BD FACSAria II provides 35- and 50-micron sample line filters to help reduce clogging of the nozzle during sample acquisition and sorting.
How can I sterilize the sample line filters?
The recommended sterilization procedure for the sample line filter is to first install it on the sample line, then run a tube of ethanol, followed by sterile deionized (DI) water. Sample line filters cannot be autoclaved and are not compatible with bleach.
How was the BD FACSAria II engineered for aerosol management?
Engineered with aerosol management in mind, the BD FACSAria II features an enclosed pathway from the sample injection chamber to the sort collection tubes.
Are there additional features for aerosol management?
For an added level of aerosol management, the BD™ Aerosol Management Option (AMO) can be added to evacuate the sort collection chamber and trap aerosolized particles during sorting.
How does the new universal mount for sort collection tubes help maintain aseptic conditions?
The new universal mount in the sort collection chamber makes inserting the tube holders easier. This design helps maintain aseptic conditions because it is easier to access the collection tubes in the tube holders.
What sizes of nozzles does the BD FACSAria II support?
Four nozzle sizes (70, 85, 100, and 130-micron) are provided to support a wide range of particle sizes.
What other improvements were made to the nozzle design?
An improved nozzle insertion design offers tight registration for a secure fit. This results in a consistent drop profile after every insertion, allowing for reproducible instrument setup. Nozzles are readily accessible and easy to change. Software enhancements include a nozzle-specific sort setup feature that matches pressure settings to the nozzle in use.
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