Frequently Asked Questions: Cytometer Setup and Tracking
What features does BD Cytometer Setup and Tracking software provide for the BD FACSAria™ II cell sorter?
BD™ Cytometer Setup and Tracking software (CS&T) automates flow cytometer setup, adjusts for instrument variability, and establishes baseline settings. These features allow you to spend less time on system setup and qualification and more time on experiments.
What is the maximum data acquisition rate of the BD FACSAria II?
Digital electronics allow the BD FACSAria II to acquire data at rates up to 70,000 events per second for up to eight parameters, with linear results over a broad dynamic range.
How does CS&T help with setup?
CS&T lets users define an instrument configuration, generate a baseline level of performance, and run subsequent performance checks. The software automatically sets up the cytometer according to this established baseline. To match the exact baseline, CS&T automatically adjusts for reproducible instrument performance.
What are the benefits of using CS&T for setup?
Using CS&T ensures the greatest possible consistency from one day to the next. It saves time and improves reliability of experiment results.
The software also allows for the creation of application-specific settings for rapid performance of routine experiments in a more consistent manner. Tracking capabilities in the software measure a number of instrument settings and provide reports on performance. Levey-Jennings graphs help users to understand instrument performance and identify maintenance issues.
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