BD OptiBuild Reagents

Frequently Asked Questions

Why was my reagent not shipped with a TDS?

In an effort to minimize our environmental footprint, we opted not to include a hardcopy of the technical data sheet (TDS) in BD OptiBuild™ products. A printable version of the TDS is available for every BD OptiBuild reagent on the product details page, or just use the Ctrl+P function.

Have all BD OptiBuild reagents been tested in a flow cytometry experiment? Is there data available to review?

Thousands of reagents were tested in flow cytometry experiments and evaluated during the development of the BD OptiBuild portfolio to ensure performance and quality. However, not every reagent batch will be tested before shipment. If you encounter any issues with reagent performance, contact our Research Applications team. If relevant data is available, they can provide and discuss to help resolve your issue.

Does BD recommend an optimal concentration for BD OptiBuild reagents?

We recommend titrating the reagent in the test setting you will be using to determine the optimal staining concentration for the specific application. We have established testing regiments to validate consistent performance, but since every reagent lot is not tested in flow, optimal concentrations will not be recommended for BD OptiBuild reagents.

Do I still need to use BD Horizon™ Brilliant Stain Buffer?

Whenever you use two or more BD Horizon Brilliant™ dyes (multiple BD OptiBuild or in combination with other BD Horizon Brilliant reagents) we recommend the use of Brilliant Stain Buffer.

I don’t see BD OptiBuild isotype controls, what should I use?

Existing BD Horizon™ isotype controls are ideal for use with BD OptiBuild reagents. The existing isotype controls were evaluated during development and found to perform effectively with all reagents.

Are BD OptiBuild reagents available for other types of dyes?

Currently BD OptiBuild reagents include BV421, BV480, BV510, BV605, BV650, BV711, BV786. BB700 and BUV395. But we’re just getting started! Visit the Future of BD OptiBuild tab

The product notice says there is free dye in these reagents. Will that affect the performance?

Known adverse effects of free dye have been evaluated and extensively tested in BD OptiBuild reagents, including in multicolor experiments, and have not been witnessed to any extent.

Will BD OptiBuild reagents be available for other cell surface markers?

We’re just getting started with BD OptiBuild reagents! We have added over 700 cell surface markers to the BD OptiBuild family and will add more as they become available. Check out the Future of BD OptiBuild tab

Are BD OptiBuild reagents available for intracellular markers?

Currently, intracellular markers will not be part of the BD OptiBuild reagent portfolio.

Why are some antibody/dye options not available?

During our development process, we identified certain antibody-dye combinations that might not perform optimally. These combinations will not be included in the portfolio as BD OptiBuild reagents. If there is interest in a particular combination, please contact your local sales representative.

I see a BD Horizon Brilliant™ reagent online but I would like to purchase the BD OptiBuild version. How can I order that?

BD OptiBuild reagents feature new antibody-dye combinations that are not currently available. As a result, the BD OptiBuild portfolio will not include any permutations that already exist in the BD catalog.

Can BD OptiBuild reagents be used for imaging?

BD OptiBuild reagents have not been tested for use in imaging. However, we have performed limited image testing on frozen mouse spleen sections and have demonstrated initial feasibility.

What buffers are recommended for staining my cells with BD OptiBuild reagents?

BD OptiBuild reagents do not require any additional considerations that are not already taken into account when determining buffers and protocols for traditional research reagents. Standard staining buffers such as Brilliant Stain Buffer BSA or Brilliant Stain Buffer FBS are recommended for use with BD OptiBuild products.

Can BD OptiBuild reagents be used with BD fix/perm buffers?

Yes, BD OptiBuild reagents are compatible with standard fix/perm buffers recommended by BD.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.