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    BV650 Rat Anti-Mouse IgE
    BV650 Rat Anti-Mouse IgE
    Flow cytometric analysis of IgE expression on 27-74 cells. Mouse 27-74 cells were stained with BV650 Rat IgG1, κ Isotype Control (Cat. No. 563848; dashed line histogram) or BV650 Rat Anti-Mouse IgE (Cat. No. 564208; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable 27-74 cells. Flow cytometric analysis was performed on a BD LSRFortessa™.
    BV650 Rat Anti-Mouse IgE
    Flow cytometric analysis of IgE expression on 27-74 cells. Mouse 27-74 cells were stained with BV650 Rat IgG1, κ Isotype Control (Cat. No. 563848; dashed line histogram) or BV650 Rat Anti-Mouse IgE (Cat. No. 564208; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable 27-74 cells. Flow cytometric analysis was performed on a BD LSRFortessa™.
    Product Details
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    BD Horizon™
    Igh-7; immunoglobulin heavy chain 7 (heavy chain of IgE)
    Mouse (QC Testing)
    Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
    Mouse IgE (pooled)
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    16020
    AB_2738669
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV650 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV650 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    7. BD Horizon Brilliant Violet 650 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
    8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
    9. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    564208 Rev. 2
    Antibody Details
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    R35-72

    The rat anti-mouse IgE antibody (clone R35-72) reacts specifically with mouse IgE of the Igh-C [a] and Igh-C [b] haplotypes. It has been reported not to react with other Ig isotypes. Detection with the rat anti-mouse IgE antibody (clone R35-72) of surface immunoglobulin on IgE-secreting hybridoma cells has also been reported.

    564208 Rev. 2
    Format Details
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    BV650
    The BD Horizon Brilliant Violet™ 650 (BV650) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 406-nm and an acceptor dye with an emission maximum (Em Max) at 649-nm. BV650, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 650-nm (e.g., a 660/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    BV650
    Violet 405 nm
    406 nm
    649 nm
    564208 Rev.2
    Citations & References
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    Development References (6)

    1. Borges MS, Kumagai Y, Okumura K, Hirayama N, Ovary Z, Tada T. Allelic polymorphism of murine IgE controlled by the seventh immunoglobulin heavy chain allotype locus. Immunogenetics. 1981; 13(6):499-507. (Biology). View Reference
    2. Driver DJ, McHeyzer-Williams LJ, Cool M, Stetson DB, McHeyzer-Williams MG. Development and maintenance of a B220- memory B cell compartment. J Immunol. 2001; 167(3):1393-1405. (Clone-specific: Flow cytometry). View Reference
    3. Kamijo S, Takeda H, Tokura T, et al. IL-33-Mediated Innate Response and Adaptive Immune Cells Contribute to Maximum Responses of Protease Allergen-Induced Allergic Airway Inflammation. J Immunol. 2013; 190(9):4489-4499. (Clone-specific: Functional assay, Stimulation). View Reference
    4. Liu FT, Albrandt K, Sutcliffe JG, Katz DH. Cloning and nucleotide sequence of mouse immunoglobulin epsilon chain cDNA. Proc Natl Acad Sci U S A. 1982; 79(24):7852-7856. (Biology). View Reference
    5. Markowitz JS, Rogers PR, Grusby MJ, Parker DC, Glimcher LH. B lymphocyte development and activation independent of MHC class II expression.. J Immunol. 1993; 150(4):1223-33. (Clone-specific: ELISA). View Reference
    6. Nishida Y, Kataoka T, Ishida N, et al. Cloning of mouse immunoglobulin epsilon gene and its location within the heavy chain gene cluster. Proc Natl Acad Sci U S A. 1981; 78(3):1581-1585. (Biology). View Reference
    View All (6) View Less
    564208 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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