Purified Mouse Anti-Rat CD1d
Clone WTH2 (also known as WTH-2) (RUO)
- Brand BD Pharmingen™
- Alternative Name Cd1d; Antigen-presenting glycoprotein CD1d; CD1d1
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG2a, κ
- Reactivity Rat (QC Testing) Mouse (Tested in Development)
Flow cytometry (Routinely Tested)
Immunohistochemistry-frozen (Tested During Development)
- Immunogen Rat CD1d Transfected Cell Line
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The WTH2 monoclonal antibody specifically binds to rat CD1d and crossreacts with mouse CD1d. CD1d is a type I transmembrane glycoprotein that is noncovalently associated with β2-microglobulin. CD1d is expressed on various cell types including subsets of thymocytes, T cells, B cells, monocytes, macrophages, dendritic cells, endothelial cells and epithelial cells. Although structurally similar to MHC Class I antigens, CD1d molecules are rather non-polymorphic and serve to present non-peptide antigens such as endogenous or microbial glycolipids to T lymphocytes (NKT cells). The WTH1 monoclonal antibody reportedly binds to a non-overlapping epitope on rat CD1d and also crossreacts with mouse CD1d when compared with WTH2. Both WTH1 and WTH2 reportedly interfere with antigen recognition by CD1d-restricted T cells.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The purified WTH2 monoclonal antibody has been reported to work for western blotting in non-reducing conditions only.