APC Mouse Anti-Mouse CD36

BD Pharmingen™ APC Mouse Anti-Mouse CD36

Name: APC Mouse Anti-Mouse CD36
Brand: BD Pharmingen™
SKU: 562744

Clone CRF D-2712

(RUO)

Multicolor flow cytometric analysis of CD36 expression on BALB/c mouse bone marrow cels. Bone marrow cells were stained simultaneously with BD Horizon™ V450 Rat anti-Mouse Ter-119 antibody (Cat. No. 560504) and with either APC Mouse IgA, κ Isotype Control (Cat. No. 562140, Left Panel) or APC Mouse anti-Mouse CD36 antibody (Cat.No. 562744, Right Panel). Two-color flow cytometric dot plots show the correlated expression patterns of CD36 (or Ig isotype control staining) versus Ter-119 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Platelet glycoprotein 4; PAS-4; GPIIIB; Fatty acid translocase; Scavenger R

Reactivity: Mouse (QC Testing)

Isotype: Mouse Cd36 null IgA, κ

Immunogen: Recombinant mouse CD36

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_2737763

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

APC Mouse IgA, κ Isotype Control RUO

Size 0.1 mg Cat No. 562140

V450 Rat Anti-Mouse TER-119/Erythroid Cells RUO

Size 50 µg Cat No. 560504

562744 Rev. 1

Antibody Details

CRF D-2712

The CRF D-2712 monoclonal antibody specifically binds to mouse and rat CD36, an 88-kDa glycoprotein member of the class B scavenger receptor family. CD36 is a multifunctional receptor that has a variety of ligands and is expressed on many cell types. Consequently, it is known by several names, including macrophage scavenger receptor, platelet glycoprotein IV, and fatty acid translocase. Cells that express CD36 include platelets, megakaryocytes, monocytes/macrophages, dendritic cells, erythroid precursors, microvascular endothelia, skeletal, cardiac, and smooth muscle cells, adipocytes, and epithelia of retina, breast, and intestine. This multi-ligand receptor functions in the clearance of apoptotic cells and abnormal erythrocytes (in malaria and sickle-cell anemia), the transport of long-chain fatty acids and oxidized low-density lipoproteins, the regulation of angiogenesis by mediating the effects of thrombospondin-1, and the mediation of coronary vasoconstriction. Crosslinking of cell-surface CD36 with plate-bound CRF D-2712 antibody promotes internalization of photoreceptor outer segment fragments by cultured retinal pigment epithelium.

562744 Rev. 1

Format Details

APC

Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: APC

Excitation Source: Red 627-640 nm

Excitation Max: 651 nm

Emission Max: 660 nm

562744 Rev.1

Citations & References

Development References (3)

Bodart V, Febbraio M, Demers A, et al. CD36 mediates the cardiovascular action of growth hormone-releasing peptides in the heart. Circ Res. 2002; 90(8):844-849. (Biology). View Reference
Febbraio M, Hajjar DP, Silverstein RL. CD36: a class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. J Clin Invest. 2001; 108(6):785-791. (Biology). View Reference
Finnemann SC, Silverstein RL. Differential roles of CD36 and alphavbeta5 integrin in photoreceptor phagocytosis by the retinal pigment epithelium. J Exp Med. 2001; 194(9):1289-1298. (Immunogen: Immunofluorescence, Immunoprecipitation). View Reference

562744 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.