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    BV421 Rat Anti-Mouse CD282 (TLR2)
    BV421 Rat Anti-Mouse CD282 (TLR2)
    Flow cytometric analysis of mouse CD282 expression by mouse spleen cells. C57BL/6 mouse splenic leucocytes were stained with PerCP-Cy™5.5 Rat Anti-Mouse CD11b antibody (Cat. No. 550993/561114) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD282 (TLR2) antibody (Cat. No. 565908; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD282 (TLR2) [or Ig Isotype control staining] versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
    BV421 Rat Anti-Mouse CD282 (TLR2)
    Flow cytometric analysis of mouse CD282 expression by mouse spleen cells. C57BL/6 mouse splenic leucocytes were stained with PerCP-Cy™5.5 Rat Anti-Mouse CD11b antibody (Cat. No. 550993/561114) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD282 (TLR2) antibody (Cat. No. 565908; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD282 (TLR2) [or Ig Isotype control staining] versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
    Product Details
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    BD Horizon™
    TLR2; Tlr2; Toll-like receptor 2; Ly105
    Mouse (QC Testing)
    Rat WI, also known as Wistar (outbred) IgG2a, κ
    Mouse TLR2 Transfected Cell Line
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    AB_2739387
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    4. Cy is a trademark of Amersham Biosciences Limited.
    5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    8. An isotype control should be used at the same concentration as the antibody of interest.
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    565908 Rev. 1
    Antibody Details
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    CB225

    The CB225 monoclonal antibody specifically binds to the mouse Toll like receptor 2 (TLR2) which is also known as, CD282. CD282 is a type 1 transmembrane glycoprotein and TLR family member. TLR2 forms heterodimers with either TLR1 or TLR6, and is expressed on monocytes, macrophages, dendritic cells, granulocytes, endothelial cells, or epithelial cells. This pattern recognition receptor is important for sensing and transducing signals in response to microbial cell wall components including lipoproteins, peptidoglycans, and lipoteichoic acid. CD282 signals through intracellular MYD88 and TRAF6 leading to NF-kappa-B activation and the production of cytokines and inflammatory mediators by responding cells. TLR2 plays a major role in mediating inflammatory and innate immune responses to many microbial pathogens.

    565908 Rev. 1
    Format Details
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    BV421
    The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV421
    Violet 405 nm
    407 nm
    423 nm
    565908 Rev.1
    Citations & References
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    Development References (5)

    1. Heine H, Kirschning CJ, Lien E, Monks BG, Rothe M, Golenbock DT. Cutting edge: cells that carry A null allele for toll-like receptor 2 are capable of responding to endotoxin. J Immunol. 1999; 162(12):6971-6975. (Biology). View Reference
    2. Matsuguchi T, Takagi K, Musikacharoen T, Yoshikai Y. Gene expressions of lipopolysaccharide receptors, toll-like receptors 2 and 4, are differently regulated in mouse T lymphocytes. Blood. 2000; 95(4):1378-1385. (Biology). View Reference
    3. Motoi Y, Shibata T, Takahashi K, et al. Lipopeptides are signaled by Toll-like receptor 1, 2 and 6 in endolysosomes.. Int Immunol. 2014; 26(10):563-73. (Immunogen: Flow cytometry, Immunoprecipitation). View Reference
    4. Shibata T, Takemura N, Motoi Y, et al. PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells. Int Immunol. 2012; 24(10):613. (Clone-specific: Flow cytometry). View Reference
    5. Underhill DM, Ozinsky A, Hajjar AM, et al. The Toll-like receptor 2 is recruited to macrophage phagosomes and discriminates between pathogens. Nature. 1999; 401(6755):811-815. (Biology). View Reference
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    565908 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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