Preparation And Storage
Recommended Assay Procedures
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The LS17-9 monoclonal antibody specifically binds to CD141, also known as thrombomodulin (TM or TRBM) or fetomodulin. CD141 is encoded by Thbd and expressed as a type I transmembrane glycoprotein with an N-terminal C-type lectin domain followed by EGF-like repeats. CD141 is expressed on a variety of cells including endothelial cells, epithelial cells, mesothelial cells, keratinocytes, synovial lining cells, fibroblastic reticular cells, megakaryocytes, dendritic cells, monocytes, macrophages, neutrophils, precursor B cells, and hematopoietic progenitor cells. CD141 is required for normal fetal development. CD141 binds thrombin and this complex can activate Protein C and Thrombin activatable fibrinolysis inhibitor (TAFI), leading to anticoagulant and antifibrinolytic pathway activities.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously.
Development References (4)
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Conway EM, Pollefeyt S, Cornelissen J, et al. Structure-function analyses of thrombomodulin by gene-targeting in mice: the cytoplasmic domain is not required for normal fetal development.. Blood. 1999; 93(10):3442-50. (Biology). View Reference
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Frontera V1, Arcangeli ML, Zimmerli C, et al. Cutting edge: JAM-C controls homeostatic chemokine secretion in lymph node fibroblastic reticular cells expressing thrombomodulin.. J Immunol. 2011; 187(2):603-607. (Immunogen: Cell separation, Flow cytometry, Fluorescence activated cell sorting, Fluorescence microscopy, Immunofluorescence, Immunoprecipitation). View Reference
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Li YH, Kuo CH, Shi GY, Wu HL. The role of thrombomodulin lectin-like domain in inflammation.. J Biomed Sci. 2012; 19:34. (Biology). View Reference
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Mionnet C, Mondor I, Jorquera A, et al. Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles.. PLoS Biol. 2013; 11(10):e1001672. (Clone-specific: Fluorescence microscopy, Immunofluorescence). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
