Purified Mouse Anti-CD100
Clone 30/CD100 (RUO)
- Brand BD Transduction Laboratories™
- Alternative Name Semaphorin 4D; Sema 4D
- Concentration 250 µg/ml
- Isotype Mouse IgG1
- Reactivity Human (QC Testing) Mouse, Rat (Tested in Development)
Western blot (Routinely Tested)
Immunofluorescence, Immunohistochemistry (Tested During Development)
Immunoprecipitation (Not Recommended)
- Immunogen Human CD100 aa. 721-861
- Storage Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
CD100 was originally described as a 150 kDa surface T-cell antigen induced after T-cell activation. Human CD100 exhibits widespread gene expression but is most prominent in peripheral lymphocytes, thymus, skeletal muscle, and brain. The full CD100 protein is 862 amino acids with homolgy to the semaphorin family of proteins at its extracellular domain followed by a short Ig-like domain. The short cytoplasmic domain contains a putative tyrosine phosphorylation site which may be the site for association with intracellular kinases. Cells that overexpress CD100 induce a rapid aggregation of B cells and increase the CD90L-B cell aggregation. Because semaphorins are axon-guiding molecules for the nervous system, it has been proposed that CD100 may have a guidance role in the immune system.
- Format Purified
Suggested Companion Products
Preparation and Storage
Store undiluted at -20°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml