PE Mouse anti-human CD142

Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info

BD Pharmingen™ PE Mouse anti-human CD142

Name: PE Mouse anti-human CD142
Brand: BD Pharmingen™
SKU: 550312

Clone HTF-1

(RUO)

LPS-stimulated peripheral blood monocytes analyzed by flow cytometry

Flow cytometric analysis of CD142 expression on activated human monocytes. Human peripheral blood mononuclear cells (PBMC) were stimulated (4 hr) with 0.1 µg/mL lipopolysaccharide (LPS; Sigma-Aldrich, Cat. No. L-2654). The cells were harvested, washed and pre-blocked for 10 min with 5% human AB serum. The cells were then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram) or PE Mouse Anti-Human CD142 antibody (Cat. No. 550312/561713; solid line histogram). The fluorescence histograms showing CD142 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact activated monocytes. Flow cytometric analysis and data analysis was performed using a BD FACSCelesta™ Flow Cytometer System and FlowJo™ software.

LPS-stimulated peripheral blood monocytes analyzed by flow cytometry

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Product Details

Brand: BD Pharmingen™

Alternative Name: Tissue factor; TF; TFA; Coagulation factor III; F3; Thromboplastin

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Human Tissue Factor Protein

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Workshop Number: VI E016, E137

Entrez Gene ID: 2152

RRID: AB_393602

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

Companion Products

PE Mouse IgG1, κ Isotype Control RUO

Size 100 Tests Cat No. 555749

550312 Rev. 7

Antibody Details

HTF-1

The HTF-1 monoclonal antibody specifically binds to CD142. CD142 is a 45-47 kDa, single chain, type I transmembrane glycoprotein also known as Tissue Factor (TF). CD142 has been referred in the literature as coagulation Factor III or thromboplastin and it is expressed on activated endothelial cells and lipopolysaccharide (LPS)-stimulated monocytes/macrophages. TF associates with factor VIIa to form a complex and acts as an enzyme that initiates the blood coagulation cascade. It is known as the major initiator of clotting in normal hemostasis. CD142 can be induced by various inflammatory mediators in monocytes and vascular endothelial cells. This antibody is useful in inflammation, thrombosis and hemostasis research.

550312 Rev. 7

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

550312 Rev.7

Citations & References

Development References (5)

Carson SD, Perry GA, Pirruccello SJ. Fibroblast tissue factor: calcium and ionophore induce shape changes, release of membrane vesicles, and redistribution of tissue factor antigen in addition to increased procoagulant activity. Blood. 1994; 84(2):526-534. (Biology). View Reference
Carson SD, Ross SE, Bach R, Guha A. An inhibitory monoclonal antibody against human tissue factor.. Blood. 1987; 70(2):490-3. (Immunogen: Dot Blot, ELISA, Flow cytometry, Inhibition, Western blot). View Reference
McComb RD, Miller KA, Carson SD. Tissue factor antigen in senile plaques of Alzheimer's disease. Am J Pathol. 1991; 139(3):491-494. (Biology). View Reference
Morrissey JH, Agis H, Albrecht S, et al. CD142 (tissue factor) Workshop Panel report. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:742-746.
Whittle SM, Yoder SC, Carson SD. Human placental tissue factor: protease susceptibility of extracellular and cytoplasmic domains. Thromb Res. 1995; 79(5-6):451-459. (Biology). View Reference

View All (5)

550312 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.