Alexa Fluor® 647 Mouse Anti-Mouse CD229
Clone Ly9.7.144 (RUO)
- Brand BD Pharmingen™
- Alternative Name Lgp100; Lymphocyte antigen 9; Ly-9; Ly9; SLAM family member 3; SLAMF3; T100
- Concentration 0.2 mg/ml
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Mouse (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Mouse Ly9 Transfected Cell Line
- Entrez Gene ID 17085
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The LY9.7.144 monoclonal antibody specifically recognizes CD229 which is also known as SLAM family member 3 (SLAMF3), Lymphocyte antigen 9 (Ly-9), Lgp100, and T100. CD229 is a ~90-120 kDa type I transmembrane glycoprotein that is encoded by Ly9 (lymphocyte antigen 9) which belongs to the Signaling Lymphocytic Activation Molecule (SLAM) family within the immunoglobulin gene superfamily. The extracellular domain of CD229 contains four extracellular Ig-like domains (2 V-set and 2 C-set) extending in order from the N-terminus as V-C2-V-C2. The intracellular region of CD229 contains two ITSMs (immunoreceptor tyrosine-based switch motifs) and serves as a docking site for SH2-binding phosphatases and adapter molecules including SAP and EAT-2. CD229 is variably expressed on most thymocytes, T cells, NKT cells, B cells, NK cells, hematopoietic stem cells and progenitor cells, and monocytes. CD229 participates in homophilic adhesion interactions between these cells and in the regulation of their activation.
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.