Two-color flow cytometric analysis of FCRL5/FCRL3 (CD307e/c) expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ V500 Mouse Anti-Human CD19 antibody (Cat. No. 561121) and either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No.558713; Left Panel) or Alexa Fluor® 647 Mouse Anti-Human FCLR5/FCLR3 (CD307e/c) antibody (Cat. No. 564343; Right Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression of CD19 versus FCLR5/FCLR3 (CD307e/c) (or Ig Isotype control staining) derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of FCRL5/FCRL3 (CD307e/c) expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ V500 Mouse Anti-Human CD19 antibody (Cat. No. 561121) and either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No.558713; Left Panel) or Alexa Fluor® 647 Mouse Anti-Human FCLR5/FCLR3 (CD307e/c) antibody (Cat. No. 564343; Right Panel). The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression of CD19 versus FCLR5/FCLR3 (CD307e/c) (or Ig Isotype control staining) derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Pharmingen™
FCRL5/FCRL3; FcRH5/FcRH3; IRTA2/IRTA3
Human (QC Testing)
Mouse IgG2b, κ
Human FCLR5 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl/test
AB_2869565
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control RUO
Size
50 Tests
Cat No.
558713
V500 Mouse anti-Human CD19 RUO
Size
100 Tests
Cat No.
561121
564343 Rev. 1
Antibody Details
307307
The 307307 monoclonal antibody specifically binds to Fc receptor-like 5 (FCLR5) and crossreacts with Fc receptor-like 3 (FCLR3). These type I transmembrane glycoproteins have sequences that are homologous to classical Fcγ receptors. FCLR5 is also known as CD307e, FCRH5 (Fc receptor homolog 5) and IRTA2 (immunoglobulin superfamily receptor translocation associated 2) whereas FCLR3 is referred to as CD307c, FCRH3 (Fc Receptor Homolog 3) and IRTA3 (immunoglobulin superfamily receptor translocation associated 3). FCRL5 and FCRL3 are differentially expressed by mature B cell subsets and play key roles in controlling signaling through the B cell receptor.
564343 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564343 Rev.1
Citations & References
Development References (8)
-
Ehrhardt GR, Cooper MD. Immunoregulatory roles for fc receptor-like molecules. Curr Top Microbiol Immunol. 201; 350:89-104. (Biology). View Reference
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Franco A, Damdinsuren B, Ise T, et al. Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors. J Immunol. 2013; 190(11):5739-5746. (Biology). View Reference
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Hatzivassiliou G, Miller I, Takizawa J, et al. IRTA1 and IRTA2, novel immunoglobulin superfamily receptors expressed in B cells and involved in chromosome 1q21 abnormalities in B cell malignancy. Immunity. 2001; 14(3):277-289. (Biology). View Reference
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Kochi Y, Myouzen K, Yamada R, et al. FCRL3, an autoimmune susceptibility gene, has inhibitory potential on B-cell receptor-mediated signaling. J Immunol. 2009; 183(9):5502-5510. (Biology). View Reference
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Maltais LJ, Lovering RC, Taranin AV, et al. New nomenclature for Fc receptor-like molecules. Nat Immunol. 2006; 7(5):431-432. (Biology). View Reference
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Nagata S, Ise T, Pastan I. Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009; 182(12):7518-7526. (Biology). View Reference
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Swainson LA, Mold JE, Bajpai UD, McCune JM. Expression of the autoimmune susceptibility gene FcRL3 on human regulatory T cells is associated with dysfunction and high levels of programmed cell death-1. J Immunol. 2010; 184(7):3639-3647. (Biology). View Reference
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Wilson TJ, Fuchs A, Colonna M. Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG. J Immunol. 2012; 188(10):4741-4745. (Biology). View Reference
564343 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
