Purified Mouse Anti-ASGPR 1
Clone 8D7 (RUO)
- Brand BD Pharmingen™
- Alternative Name Asialoglycoprotein receptor 1, ASGR1, ASGP-R1, ASGPR 1, HL-1, CLEC4H1
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing) Rat (Reported)
Flow cytometry (Routinely Tested)
Bioimaging, Immunofluorescence (Tested During Development)
Western blot (Not Recommended)
- Immunogen Rat Liver membrane constituents
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 8D7 monoclonal antibody recognizes Asialoglycoprotein receptor 1 (ASGPR 1), also known as Hepatic lectin H1 (HL-1). ASGPR 1 is an approximately 42 kDA type II integral membrane protein that is expressed on the surface of hepatic cells. It is expressed by hepatocytes on the sinusoidal-lateral plasma membrane but not on the bile canalicular membrane. ASGPR 1 plays a role in serum glycoprotein homeostasis. It functions as a subunit of the Asialoglycoprotein receptor (ASGPR) complex that binds, internalizes, and transports various glycoproteins for lysosomal degradation. The receptor may also promote hepatic infection by the binding and uptake of various viruses. The immunogen used to generate the 8D7 hybridoma was rat liver membrane extracts. Rat ASGPR consists of three polypeptide subunits (Rat hepatic lectin 1-3 (RHL1-3). The 8D7 antibody has been shown to react with a subunit-specific epitope on RHL-1. Clone 8D7 cross-reacts with human ASGPR 1.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Accutase is a registered trademark of Innovative Cell Technologies, Inc.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.