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    Purified Mouse Anti-ASGPR 1
    Purified Mouse Anti-ASGPR 1
    Flow Cytometric Analysis of ASGPR 1 expression in Human Hepatocellular Carcinoma (Hep G2) cells. Hep G2 cells (ATCC, HB-8065) were harvested using BD™ Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; dashed line histogram) or Purified Mouse Anti-ASGPR 1 antibody (Cat. No. 563654; solid line histogram) at matched concentrations. The second-step reagent was PE Goat Anti-Mouse Ig (Cat. No. 550589). Histograms were derived from gated events based on light-scattering characteristics of viable Hep G2 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometry System.
    Purified Mouse Anti-ASGPR 1
    Immunofluorescent Staining of ASGPR 1 in Human Hepatocellular Carcinoma (Hep G2) cells. Hep G2 cells (ATCC, HB-8065) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and stained with Purified Mouse Anti-ASGPR 1 (pseudo-colored green) at 5 μg/mL. The second-step reagent was Alexa Fluor® 488 Goat Anti-Mouse IgG (Life Technologies), and counter-staining was with DAPI (4',6-Diamidino-2-phenylindole dihydrochloride; pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.
    Purified Mouse Anti-ASGPR 1 Purified Mouse Anti-ASGPR 1
    Flow Cytometric Analysis of ASGPR 1 expression in Human Hepatocellular Carcinoma (Hep G2) cells. Hep G2 cells (ATCC, HB-8065) were harvested using BD™ Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; dashed line histogram) or Purified Mouse Anti-ASGPR 1 antibody (Cat. No. 563654; solid line histogram) at matched concentrations. The second-step reagent was PE Goat Anti-Mouse Ig (Cat. No. 550589). Histograms were derived from gated events based on light-scattering characteristics of viable Hep G2 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometry System.
    Immunofluorescent Staining of ASGPR 1 in Human Hepatocellular Carcinoma (Hep G2) cells. Hep G2 cells (ATCC, HB-8065) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and stained with Purified Mouse Anti-ASGPR 1 (pseudo-colored green) at 5 μg/mL. The second-step reagent was Alexa Fluor® 488 Goat Anti-Mouse IgG (Life Technologies), and counter-staining was with DAPI (4',6-Diamidino-2-phenylindole dihydrochloride; pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.
    Product Details
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    BD Pharmingen™
    Asialoglycoprotein receptor 1, ASGR1, ASGP-R1, ASGPR 1, HL-1, CLEC4H1
    Human (QC Testing), Rat (Reported)
    Mouse BALB/c IgG1, κ
    Rat Liver membrane constituents
    Flow cytometry (Routinely Tested), Bioimaging, Immunofluorescence (Tested During Development), Western blot (Not Recommended)
    0.5 mg/ml
    AB_2687909
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Accutase is a registered trademark of Innovative Cell Technologies, Inc.
    6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    563654 Rev. 1
    Antibody Details
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    8D7

    The 8D7 monoclonal antibody recognizes Asialoglycoprotein receptor 1 (ASGPR 1), also known as Hepatic lectin H1 (HL-1).  ASGPR 1 is an approximately 42 kDA type II integral membrane protein that is expressed on the surface of hepatic cells. It is expressed by hepatocytes on the sinusoidal-lateral plasma membrane but not on the bile canalicular membrane. ASGPR 1 plays a role in serum glycoprotein homeostasis. It functions as a subunit of the Asialoglycoprotein receptor (ASGPR) complex that binds, internalizes, and transports various glycoproteins for lysosomal degradation. The receptor may also promote hepatic infection by the binding and uptake of various viruses. The immunogen used to generate the 8D7 hybridoma was rat liver membrane extracts. Rat ASGPR consists of three polypeptide subunits (Rat hepatic lectin 1-3 (RHL1-3). The 8D7 antibody has been shown to react with a subunit-specific epitope on RHL-1. Clone 8D7 cross-reacts with human ASGPR 1.

                      

    563654 Rev. 1
    Format Details
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    Purified
    Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
    Purified
    563654 Rev.1
    Citations & References
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    Development References (3)

    1. Mizuno M, Yamada G, Nagashima H. Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. J Gastroenterol. 1986; 21(3):238-244. (Immunogen). View Reference
    2. Shimada M, Mizuno M, Uesu T, et al. A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res. 2003; 26(1):55-60. (Clone-specific: Flow cytometry, Immunofluorescence, Immunohistochemistry). View Reference
    3. Touboul T, Hannan NR, Corbineau S, et al. Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development. Hepatology. 2010; 51(5):1754-1765. (Biology: Array). View Reference
    563654 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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