CD71 APC

BD™ CD71 APC

Name: CD71 APC
Brand: BD™
SKU: 337447

Clone L01.1

(CE_IVD)

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Product Details

Brand: BD™

Alternative Name: TFR; TFRC; Trfr; TfR1; Transferrin receptor protein 1

Reactivity: Human

Isotype: Mouse BALB/c IgG2a

Immunogen: Pokeweed–stimulated peripheral blood cells

Application: Flow cytometry

Concentration: 25 μg/mL

Vol. Per Test: 5 μL

Workshop Number: II L-13

Entrez Gene ID: 7037

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: CE_IVD

Preparation And Storage

The antibody reagent is stable until the expiration date shown on the label when stored at 2°C to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.

Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.

337447 Rev. 1

Antibody Details

L01.1

CD71 (Anti-Transferrin Receptor) is intended for in vitro diagnostic use in the identification of cells expressing CD71 antigen, using a BD FACS™ brand flow cytometer. The flow cytometer must be equipped to detect light scatter and the appropriate fluorescence, and be equipped with appropriate analysis software for data acquisition and analysis. Refer to your instrument user’s guide for instructions.

337447 Rev. 1

Format Details

APC

Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: APC

Excitation Source: Red 627-640 nm

Excitation Max: 651 nm

Emission Max: 660 nm

337447 Rev.1

Citations & References

Development References (18)

Blair A, Hogge DE, Sutherland HJ. Most acute myeloid leukemia progenitor cells with long-term proliferative ability in vitro and in vivo have the phenotype CD3(+)/CD71(–)/HLA-DR–. Blood. 1998; 92:4325-4335. (Biology).
Braylan RC, Orfao A, Borowitz MJ, Davis BH. Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting. Clin Cytometry. 2001; 46:23-27. (Biology).
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Lymphocytes: Approved Guideline. NCCLS document H42-A. 1998. (Biology).
Consensus protocol for the flow cytometric immunophenotyping of hematopoietic malignancies. Rothe G, Schmitz G. Leukemia. 1996; 10:877-895. (Biology).
Jackson AL, Warner NL. Rose NR, Friedman H, Fahey JL, ed. Manual of Clincial Laboratory Immunology, Third Edition. Washington DC: American Society for Microbiology; 1986:226-235.
Jefferies WA, Brandon MR, Hunt SV, Williams AF, Gatter KC, Mason DY. Transferrin receptor on endothelium of brain capillaries. Nature. 1998; 312(5990):162-163. (Biology). View Reference
Judd W, Poodry CA, Strominger JL. Novel surface antigens expressed on dividing cells but absent from non-dividing cells. J Exp Med. 1980; 152:1430. (Biology).
Knowles DM. Knowles DM, Thompson DD, ed. Neoplastic Hematopathology. Philadelphia, PA: Williams & Wilkins; 2001.
Larrick JW, Cresswell P. Modulation of cell surface iron transferrin receptors by cellular density and state of activation.. J Supramol Struct. 1979; 11(4):579-86. (Biology). View Reference
Loken MR, Shah VO, Dattilio K, Civin C. Flow cytometric analysis of human bone marrow, I: Normal erythroid development. Blood. 1987; 69:255-263. (Biology).
Maynadie M, Picard F, Husson B, et al. Immunophenotypic clustering of myelodysplastic syndromes. Blood. 2002; 100:2349-2356. (Biology).
McClosky TW, Oyaizu N, Pahwa S. Review: Apoptosis, HIV infection, and the Fas antigen. Clin Immunol Newsletter. 1995; 15:105-113. (Biology).
NCCLS document. 2001. (Biology).
Newman R, Schneider C, Sutherland R, Vodinelish L, Greaves M. The transferrin receptor. Trends Biochem Sci. 1982; 1:397. (Biology).
Phillips JH, Le AM, Lanier LL. Natural killer cells activated in a human mixed lymphocyte response culture identified by expression of Leu-11 and class II histocompatibility antigens.. J Exp Med. 1984; 159(4):993-1008. (Biology). View Reference
Schwarting R, Stein H. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:455-460.
Stelzer GT, Marti G, Hurley A, McCoy PJ, Lovett EJ, Schwartz A. US-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry. 1997; 30:214-230. (Biology).

View All (18)

337447 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For In Vitro Diagnostic Use.

23-22942-00

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