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BV605 Mouse Anti-Human Integrin αvβ6
Product Details
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BD OptiBuild™
αVβ6; avb6; CD51/β6; ITGAV/ITGB6; Integrin alpha v beta 6
Human (Tested in Development)
Mouse 129 x C57BL/6 IgG2a
129/C57BL/6 β6 -/- Mice were mmunized with Human αvβ6 or Mouse Keratinocytes
Flow cytometry (Qualified)
0.2 mg/ml
AB_2875793
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  2. Researchers should determine the optimal concentration of this reagent for their individual applications.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  9. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  10. CF™ is a trademark of Biotium, Inc.
  11. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from BD Horizon™ BV421 may be observed. Therefore, we recommend that individual compensation controls be performed for every BD Horizon™ BV605 conjugate.
  12. BD Horizon Brilliant Violet 605 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
751821 Rev. 1
Antibody Details
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10D5

The 10D5 monoclonal antibody specifically recognizes Integrin alpha v beta 6 (αvβ6) that is also known as CD51/β6. This heterodimeric receptor is comprised of transmembrane type I alpha and beta chain glycoproteins that are encoded by ITGAV (integrin subunit alpha) and ITGB6 (integrin subunit beta 6), respectively. Like other integrins, αvβ6 serves as a transmembrane adhesion receptor that can tether the intracellular cytoskeleton to the extracellular matrix (ECM) by binding to R-G-D sequences of ECM protein ligands including fibronectin, vitronectin, and tenascin. Integrin αvβ6 likewise plays a key role in the activation of transforming growth factor beta-1 (TGFβ1) by its R-G-D-dependent release of TGFβ1 from regulatory Latency-associated peptide (LAP). Some viruses, such as. Coxsackieviruses and Herpes simplex virus-1 use Integrin αvβ6 as a receptor to infect cells. Integrin αvβ6 engages in bidirectional signaling, ie, outside-in and inside-out signaling, to regulate essential cellular functions. These include adhesion, migration, polarization, differentiation, and proliferation that are crucial to tissue homeostasis. Although not normally expressed on adult epithelial cells/keratinocytes, its expression is upregulated on these cells during the course of embryonic development, inflammation, tissue injury, wound healing, as well as malignant transformation. The 10D5 antibody blocks Integrin αvβ6-dependent cellular adhesion and migration and reportedly recognizes both human and mouse αvβ6 integrins. Binding of 10D5 antibody to αvβ6 is divalent-cation dependent.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

751821 Rev. 1
Format Details
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BV605
The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm. BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter). The acceptor dye can be excited by the yellow-green (561-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV605
Violet 405 nm
407 nm
605 nm
751821 Rev.1
Citations & References
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Development References (14)

  1. Allen MD, Thomas GJ, Clark S, et al. Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of αvβ6 integrin in DCIS identifies high-risk patients and predicts recurrence.. Clin Cancer Res. 2014; 20(2):344-57. (Clone-specific: Blocking, Flow cytometry, Functional assay). View Reference
  2. Bandyopadhyay A, Raghavan S. Defining the role of integrin alphavbeta6 in cancer.. Curr Drug Targets. 2009; 10(7):645-52. (Biology). View Reference
  3. Bates RC, Bellovin DI, Brown C, et al. Transcriptional activation of integrin beta6 during the epithelial-mesenchymal transition defines a novel prognostic indicator of aggressive colon carcinoma. J Clin Invest. 2005; 115(2):339-47. (Clone-specific: Blocking). View Reference
  4. Huang X, Wu J, Spong S, Sheppard D. The integrin alphavbeta6 is critical for keratinocyte migration on both its known ligand, fibronectin, and on vitronectin. J Cell Sci. 1998; 111(15):2189-95. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation, Inhibition). View Reference
  5. Jackson T, Sheppard D, Denyer M, Blakemore W, King AM. The epithelial integrin αvβ6 is a receptor for foot-and-mouth disease virus. J Virol. 2000; 74(11):4949-56. (Clone-specific: Blocking). View Reference
  6. Jovanovic J, Takagi J, Choulier L, et al. alphaVbeta6 is a novel receptor for human fibrillin-1. Comparative studies of molecular determinants underlying integrin-rgd affinity and specificity.. J Biol Chem. 2007; 282(9):6743-51. (Clone-specific: Blocking, Flow cytometry). View Reference
  7. Koivisto L, Bi J, Häkkinen L, Larjava H. Integrin αvβ6: Structure, function and role in health and disease.. Int J Biochem Cell Biol. 2018; 99:186-196. (Biology). View Reference
  8. Li X, Yang Y, Hu Y, et al. Alphavbeta6-Fyn signaling promotes oral cancer progression.. J Biol Chem. 2003; 278(43):41646-53. (Clone-specific: Blocking). View Reference
  9. Maier S, Paulsson M, Hartmann U. The widely expressed extracellular matrix protein SMOC-2 promotes keratinocyte attachment and migration.. Exp Cell Res. 2008; 314(13):2477-87. (Clone-specific: Blocking). View Reference
  10. Miller LC, Blakemore W, Sheppard D, Atakilit A, King AM, Jackson T. Role of the cytoplasmic domain of the beta-subunit of integrin alpha(v)beta6 in infection by foot-and-mouth disease virus. J Virol. 2001; 75(9):4158-64. (Clone-specific: Blocking). View Reference
  11. Munger JS, Huang X, Kawakatsu H, et al. The integrin alpha v beta 6 binds and activates latent TGF beta 1: a mechanism for regulating pulmonary inflammation and fibrosis.. Cell. 1999; 96(3):319-28. (Clone-specific: Blocking, Functional assay). View Reference
  12. Thomas GJ, Lewis MP, Whawell SA, et al. Expression of the alphavbeta6 integrin promotes migration and invasion in squamous carcinoma cells.. J Invest Dermatol. 2001; 117(1):67-73. (Clone-specific: Blocking). View Reference
  13. Thomas GJ, Nyström ML, Marshall JF. Alphavbeta6 integrin in wound healing and cancer of the oral cavity.. J Oral Pathol Med. 2006; 35(1):1-10. (Biology). View Reference
  14. Williams CH, Kajander T, Hyypiä T, Jackson T, Sheppard D, Stanway G. Integrin alpha v beta 6 is an RGD-dependent receptor for coxsackievirus A9. J Virol. 2004; 78(13):6967-73. (Clone-specific). View Reference
View All (14) View Less
751821 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.