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BV421 Rat Anti-Mouse CRIg
BV421 Rat Anti-Mouse CRIg
Flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse CRIg antibody (Cat. No. 749507; solid line histogram) on BALB/c mouse peritoneal macrophages, with corresponding Isotype Control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse CRIg antibody (Cat. No. 749507; solid line histogram) on BALB/c mouse peritoneal macrophages, with corresponding Isotype Control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Product Details
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BD OptiBuild™
CRIg; Vsig4; Z39IG
Mouse (Tested in Development)
Rat IgG2a, κ
Mouse CRIg Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
278180
AB_2873875
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
749507 Rev. 3
Antibody Details
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17C9

The 17C9 monoclonal antibody specifically recognizes Complement receptor of the immunoglobulin superfamily (CRIg) which is encoded by the Vsig4 (V-set and immunoglobulin domain containing 4) gene. CRIg is a ~45 kDa single-pass type I transmembrane glycoprotein that contains a variable (V-type) immunoglobulin domain in its extracellular region which is required for binding complement components and promoting phagocytosis. CRIg is expressed on Kupffer cells and tissue resident macrophages, including a subset of resident peritoneal macrophages. CRIg binds complement fragments C3b and iC3b and plays important roles in innate and adaptive immunity. This complement receptor is involved in regulation of the complement cascade and serves as a phagocytic receptor in the clearance of pathogens, immune complexes, and apoptotic cells. CRIg-positive macrophages can reportedly function in the negative regulation of T-cell proliferation and IL-2 production.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

749507 Rev. 3
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
749507 Rev.3
Citations & References
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Development References (4)

  1. Fu W, Wojtkiewicz G, Weissleder R, Benoist C, Mathis D. Early window of diabetes determinism in NOD mice, dependent on the complement receptor CRIg, identified by noninvasive imaging. Nat Immunol. 2012; 13(4):361-368. (Clone-specific). View Reference
  2. Helmy KY, Katschke KJ, Jr., et al. CRIg: a macrophage complement receptor required for phagocytosis of circulating pathogens. Cell. 2006; 124(5):915-927. (Biology). View Reference
  3. Small AG, Al-Baghdadi M, Quach A, Hii C, Ferrante A. Complement receptor immunoglobulin: a control point in infection and immunity, inflammation and cancer. Swiss Med Wkly. 2016; 146:w14301. (Biology). View Reference
  4. Vogt L, Schmitz N, Kurrer MO, et al. VSIG4, a B7 family-related protein, is a negative regulator of T cell activation. J Clin Invest. 2006; 116(10):2817-2826. (Biology). View Reference
View All (4) View Less
749507 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.