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BV750 Hamster Anti-Mouse Vδ 6.3/2 TCR
Product Details
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BD OptiBuild™
V delta 6.3/2 TCR; TCR Vδ 6.3/2; TCR Vδ6.3/2; TCR Vd6.3/2
Mouse (Tested in Development)
Armenian Hamster IgG2, κ
1E6.11.D7, a C57BL/6 mouse Vγ1.1Vδ6.3 TCR-bearing IEL/T-cell hybridoma
Flow cytometry (Qualified)
0.2 mg/ml
AB_2872067
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV750 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant™ Violet 750 is covered by one or more of the following US patents: 8,158,444; 8,802,450; 8,575,303; 8,455,613; 8,227,187; 8,841,072; 8,110,673.
747372 Rev. 2
Antibody Details
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8F4H7B7

The 8F4H7B7 antibody reacts with Vδ 6.3/2 T-cell Receptor (TCR)-bearing T lymphocytes.  Originally defined as a member of the Vδ 6 TCR subfamily, it is now proposed that the C57BL-derived Vδ 6.3 is an allelic variant of Vδ 6.2, found in A/J, AKR, BALB/c, C3H/He, and FVB mice.  mAb 8F4H7B7 crossreacts with Vδ 6.4 and possibly Vδ 6.6 in DBA/2 mice, and it also detects a subset of γδ TCR-bearing cells in CBA/J and C57L mice.  It does not recognize Vδ 4, Vδ 5, Vδ 6.1, or Vδ 6.5 TCR.  A subpopulation of thymocytes expressing Vδ 6.3 or Vδ 6.4 TCR (in C57BL/6 or DBA/2 mice, respectively) and low levels of CD90.2 (Thy-1.2) shares functional and phenotypic characteristics with NK-T cells.  Similar δV 6.4 TCRexpressing lymphocytes make up significant proportions of the γδ T-cell populations in the liver and spleen of DBA/2 mice. Furthermore, T lymphocytes bearing Vδ 6.3/2 TCR are found in the skin and intestinal epithelium and may represent a unique T-cell subpopulation with a potential for autoimmune reactivity.

The antibody was conjugated to BD Horizon™ BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 750-nm. BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass. Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended.

747372 Rev. 2
Format Details
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BV750
The BD Horizon Brilliant Violet™ 750 (BV750) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 409-nm and an acceptor dye with an emission maximum (Em Max) at 754-nm. BV750, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 750-nm (e.g., a 750/30 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV750
Violet 405 nm
409 nm
754 nm
747372 Rev.2
Citations & References
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Development References (5)

  1. Azuara V, Grigoriadou K, Lembezat MP, Nagler-Anderson C, Pereira P. Strain-specific TCR repertoire selection of IL-4-producing Thy-1 dull gamma delta thymocytes. Eur J Immunol. 2001; 31(1):205-214. (Immunogen). View Reference
  2. Azuara V, Lembezat MP, Pereira P. The homogeneity of the TCRdelta repertoire expressed by the Thy-1dull gammadelta T cell population is due to cellular selection. Eur J Immunol. 1998; 28(11):3456-3467. (Biology). View Reference
  3. Gerber DJ, Azuara V, Levraud JP, Huang SY, Lembezat MP, Pereira P. IL-4-producing gamma delta T cells that express a very restricted TCR repertoire are preferentially localized in liver and spleen. J Immunol. 1999; 163(6):3076-3082. (Biology). View Reference
  4. Kalataradi H, Eyster CL, Fry A. Allelic differences in TCR gamma-chains alter gamma delta T cell antigen reactivity. J Immunol. 1994; 153(4):1455-1465. (Biology). View Reference
  5. Nagler-Anderson C, McNair LA, Cradock A. Self-reactive, T cell receptor-gamma delta+, lymphocytes from the intestinal epithelium of weanling mice. J Immunol. 1992; 149(7):2315-2322. (Biology). View Reference
View All (5) View Less
747372 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.