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BV510 Mouse Anti-Human Nectin-1 (CD111)
Product Details
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BD OptiBuild™
Nectin1; PRR1; PVRL1; PVRR1; HveC; HIgR; CLPED1; ED4; SK-12; OFC7
Human (Tested in Development)
Mouse IgG1, κ
Human Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2742676
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
745054 Rev. 1
Antibody Details
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CK41

The CK41 monoclonal antibody specifically binds to Nectin-1, which is also known as, CD111, Poliovirus receptor-related protein 1(PRR1, PVRL1), Herpes virus entry mediator C (HveC), or Herpesvirus Ig-like receptor (HIgR). Nectin-1 is a type I transmembrane glycoprotein that belongs to the Ig gene superfamily. CD111 is expressed on a variety of cell types including, epithelial cells, endothelial cells, neuronal cells, megakaryocytes, and CD34 positive stem cells. CD111 functions as a receptor for herpes simplex virus 1 (HSV 1) and HSV 2.  Nectin-1 also serves as an intercellular adhesion molecule helping to form junctions between cells, eg, between endothelial cells or epithelial cells. Nectin-1 can bind to other Nectin-1 molecules and Nectin family members and to CD155 (Poliovirus Receptor/PVR).

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

745054 Rev. 1
Format Details
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BV510
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV510
Violet 405 nm
327 nm, 405 nm
512 nm
745054 Rev.1
Citations & References
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Development References (4)

  1. Krummenacher C, Baribaud I, Eisenberg RJ, Cohen GH. Cellular localization of nectin-1 and glycoprotein D during herpes simplex virus infection. J Virol. 2003; 77(16):8985-8999. View Reference
  2. Krummenacher C1, Baribaud I, Ponce de Leon M, et al. Localization of a binding site for herpes simplex virus glycoprotein D on herpesvirus entry mediator C by using antireceptor monoclonal antibodies. J Virol. 2000; 74(23):10863-10872. View Reference
  3. Richart SM1, Simpson SA, Krummenacher C, et al. Entry of herpes simplex virus type 1 into primary sensory neurons in vitro is mediated by Nectin-1/HveC. J Virol. 2003; 77(5):3307-3311. View Reference
  4. Simpson SA, Manchak MD, Hager EJ, et al. Nectin-1/HveC Mediates herpes simplex virus type 1 entry into primary human sensory neurons and fibroblasts. 2005; 11(2):208-218. View Reference
View All (4) View Less
745054 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.