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    BV421 Mouse Anti-Human Podoplanin
    BV421 Mouse Anti-Human Podoplanin
    Flow cytometric analysis of human podoplanin expression on human teratocarcinoma cell line. NCCIT cells (ATCC CRL-2073) were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; 0.5 µg, dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human Podoplanin (Cat. No. 566456; 0.5 µg, solid line histogram). The fluorescence histogram showing podoplanin expression (or Ig Isotype control staining), were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
    BV421 Mouse Anti-Human Podoplanin
    Immunohistofluorescent staining of Podoplanin in human placenta. The acetone-fixed sections of frozen tissue were stained with BD Horizon™ BV421 Mouse Anti-Human Podoplanin (Cat. No. 566456, pseudocolored red) and DRAQ5™ (Cat. No. 564902 or 564903), pseudocolored green) at 0.5 µg. Photography was performed on a Molecular Devices standard epifluorescence microscope. Original magnification, 20×. Data shown on this Technical Data Sheet are not lot specific.
    BV421 Mouse Anti-Human Podoplanin BV421 Mouse Anti-Human Podoplanin
    Flow cytometric analysis of human podoplanin expression on human teratocarcinoma cell line. NCCIT cells (ATCC CRL-2073) were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; 0.5 µg, dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human Podoplanin (Cat. No. 566456; 0.5 µg, solid line histogram). The fluorescence histogram showing podoplanin expression (or Ig Isotype control staining), were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD FACSCanto™II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
    Immunohistofluorescent staining of Podoplanin in human placenta. The acetone-fixed sections of frozen tissue were stained with BD Horizon™ BV421 Mouse Anti-Human Podoplanin (Cat. No. 566456, pseudocolored red) and DRAQ5™ (Cat. No. 564902 or 564903), pseudocolored green) at 0.5 µg. Photography was performed on a Molecular Devices standard epifluorescence microscope. Original magnification, 20×. Data shown on this Technical Data Sheet are not lot specific.
    Product Details
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    BD Horizon™
    PDPN; T1-alpha (T1A); GP36; GP40; OTS8; T1A2; TI1A; T1A-2; AGGRUS; PA2.26
    Human (QC Testing)
    Mouse BALB/c IgG1, κ
    Human PDPN Transfected Cell Line
    Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Reported)
    0.2 mg/ml
    10630
    AB_2739735
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD Optibuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    7. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
    8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
    9. DRAQ5™ is a registered trademark of BioStatus Ltd.
    10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    566456 Rev. 2
    Antibody Details
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    LpMab-17

    The LpMab-17 monoclonal antibody specifically recognizes human podoplanin which is encoded by PDPN. Podoplanin is a 38-44 kDa type I transmembrane glycoprotein. This heavily glycosylated mucin type protein is named for its expression on kidney glomerular epithelial cells known as podocytes. It is also expressed on a wide variety of cell types, including intestinal and thymic epithelial cells, alveolar type I cells, fibroblastic reticular cells, lymphatic endothelial cells, osteoblasts, and Th17 cells. Its expression is upregulated in some tumors. Podoplanin induces platelet aggregation via its three platelet aggregation-stimulating (PLAG) domains; it binds C-type lectin domain family 1 member B (Clec1B, also known as CLEC2); and it is involved in actin cytoskeleton organization and in cellular adhesion and migration. It also plays roles in organogenesis, vascular development, inflammatory diseases, tumorigenesis, and cancer cell motility and metastasis.

    The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes.  With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

    566456 Rev. 2
    Format Details
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    BV421
    The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV421
    Violet 405 nm
    407 nm
    423 nm
    566456 Rev.2
    Citations & References
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    Development References (7)

    1. Astarita JL, Acton SE, Turley SJ. Podoplanin: emerging functions in development, the immune system, and cancer.. Front Immunol. 2012; 3:283. (Biology). View Reference
    2. Chang JE, Turley SJ. Stromal infrastructure of the lymph node and coordination of immunity. Trends Immunol.. 2015; 36(1):30-9. (Biology). View Reference
    3. Dang Q, Liu J, Li J, Sun Y. Podoplanin: a novel regulator of tumor invasion and metastasis. Med Oncol. 2014; 31(9):24. (Biology). View Reference
    4. Kato Y, Ogasawara S, Oki H, et al. Novel Monoclonal Antibody LpMab-17 Developed by CasMab Technology Distinguishes Human Podoplanin from Monkey Podoplanin. Monoclon Antib Immunodiagn Immunother. 2016; 35(2):109-16. (Immunogen: Flow cytometry, Immunohistochemistry, Western blot). View Reference
    5. Pan Y, Xia L. Emerging roles of podoplanin in vascular development and homeostasis. Front Med. 2015; 9(4):421-30. (Biology). View Reference
    6. Retzbach EP, Sheehan SA, Nevel EM, et al. Podoplanin emerges as a functionally relevant oral cancer biomarker and therapeutic target. j.oraloncology. 2018; 78:126-136. (Biology). View Reference
    7. Ugorski M, Dziegiel P, Suchanski J. Podoplanin - a small glycoprotein with many faces. Am J Cancer Res. 2016; 6(2):370-86. (Biology). View Reference
    View All (7) View Less
    566456 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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