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    BV421 Mouse Anti-Human CXCR6 (CD186)
    BV421 Mouse Anti-Human CXCR6 (CD186)
    Two-color flow cytometric analysis of CXCR6 (CD186) expression on human peripheral blood cells. Human peripheral blood cells were freshly stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555342/561804) and either BD Horizon™ BV421 Anti-Human CXCR6 (CD186) (Cat. No. 566007/566008) or BD Horizon BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color contour plots showing the correlated expression of CXCR6 (CD186) [or Ig Isotype control staining] versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.
    BV421 Mouse Anti-Human CXCR6 (CD186)
    Flow cytometric analysis of CXCR6 (CD186) expression on IL-2-stimulated lymphocytes. Human PBMC were cultured (9 days) with Recombinant Human IL-2 (Cat. No. 554603). Cells were stained with BD Horizon Fixable Viability Stain 660 (FVS660; Cat. No. 564405) for exclusion of dead cells and then with either BD Horizon BV421 Mouse IgG2a, κ Isotype Control (dashed line histogram) or BD Horizon BV421 Anti-Human CXCR6 (CD186) antibody (solid line histogram).  The histograms showing CXCR6 (CD186) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable FVS660-negative lymphocytes using a BD LSRFortessa™ X-20 Cell Analyzer System.
    BV421 Mouse Anti-Human CXCR6 (CD186) BV421 Mouse Anti-Human CXCR6 (CD186)
    Two-color flow cytometric analysis of CXCR6 (CD186) expression on human peripheral blood cells. Human peripheral blood cells were freshly stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 555342/561804) and either BD Horizon™ BV421 Anti-Human CXCR6 (CD186) (Cat. No. 566007/566008) or BD Horizon BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color contour plots showing the correlated expression of CXCR6 (CD186) [or Ig Isotype control staining] versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.
    Flow cytometric analysis of CXCR6 (CD186) expression on IL-2-stimulated lymphocytes. Human PBMC were cultured (9 days) with Recombinant Human IL-2 (Cat. No. 554603). Cells were stained with BD Horizon Fixable Viability Stain 660 (FVS660; Cat. No. 564405) for exclusion of dead cells and then with either BD Horizon BV421 Mouse IgG2a, κ Isotype Control (dashed line histogram) or BD Horizon BV421 Anti-Human CXCR6 (CD186) antibody (solid line histogram).  The histograms showing CXCR6 (CD186) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable FVS660-negative lymphocytes using a BD LSRFortessa™ X-20 Cell Analyzer System.
    Product Details
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    BD Horizon™
    C-X-C chemokine receptor type 6; BONZO; STRL33; TYMSTR
    Human (QC Testing)
    Mouse BALB/c IgG2a, κ
    Human CXCR6
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    10663
    AB_2744472
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    566008 Rev. 1
    Antibody Details
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    13B 1E5

    The 13B 1E5 monoclonal antibody specifically binds to C-X-C chemokine receptor type 6 (CXCR6), which is also known as CD186, BONZO, T-lymphocyte-expressed seven-transmembrane domain receptor (TYMSTR), and Seven transmembrane receptor-like from clone 33 (STRL33). CXCR6 is a G-protein coupled chemokine receptor that is expressed on subsets of activated and memory T cells and NKT cells. CXCR6 binds to soluble CXCL16 and membrane-anchored CXCL16 expressed by dendritic cells and macrophages. The CXCL16 and CXCR6 interaction activates Akt and mTor signaling. This regulates cellular migration including the recruitment of tumor-infiltrating lymphocytes and may contribute to the progression and metastasis of various cancers. CXCR6 also can serve as a coreceptor for certain strains of HIV-1 and HIV-2.

    The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

    566008 Rev. 1
    Format Details
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    BV421
    The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV421
    Violet 405 nm
    407 nm
    423 nm
    566008 Rev.1
    Citations & References
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    Development References (6)

    1. Deng HK, Unutmaz D, KewalRamani VN, Littman DR. Expression cloning of new receptors used by simian and human immunodeficiency viruses.. Nature. 1997; 388(6639):296-300. (Biology). View Reference
    2. La Porta CA. CXCR6: the role of environment in tumor progression. Challenges for therapy.. Stem Cell Rev. 2012; 8(4):1282-5. (Biology). View Reference
    3. Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber JM. STRL33, A novel chemokine receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic HIV-1.. J Exp Med. 1997; 185(11):2015-23. (Biology). View Reference
    4. Loetscher M, Amara A, Oberlin E, et al. TYMSTR, a putative chemokine receptor selectively expressed in activated T cells, exhibits HIV-1 coreceptor function.. Curr Biol. 1997; 7(9):652-60. (Biology). View Reference
    5. Murphy PM. International Union of Pharmacology. XXX. Update on chemokine receptor nomenclature.. Pharmacol Rev. 2002; 54(2):227-9. (Biology). View Reference
    6. Xiao G, Wang X, Wang J, et al. CXCL16/CXCR6 chemokine signaling mediates breast cancer progression by pERK1/2-dependent mechanisms.. Oncotarget. 2015; 6(16):14165-78. (Biology). View Reference
    View All (6) View Less
    566008 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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