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    PE Rat Anti-Mouse CD268 (BAFF-R)
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    This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
    PE Rat Anti-Mouse CD268 (BAFF-R)
    Multicolor flow cytometric analysis of mouse CD268 (BAFF-R) expression.      Left Panel - Spleen. BALB/c splenic leucocytes were stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553092/561880), and either PE Rat IgG1, κ Isotype Control (Cat. No. 554685; Left Plot), or PE Rat Anti-Mouse CD268 (BAFF-R) antibody (Cat. No. 565783; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD268 (BAFF-R) [or Ig Isotype control staining] versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes.      Right Panel: Bone Marrow. BALB/c bone marrow cells were stained with APC Rat Anti-Mouse CD45R/B220 and BD Horizon™ BUV395 Rat Anti-Mouse IgM (Cat. No. 564025) antibodies, and either PE Rat IgG1, κ Isotype Control (Left Plot) or PE Rat Anti-Mouse CD268 (BAFF-R) antibody (Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD268 (BAFF-R) [or Ig Isotype control staining] versus IgM were derived from CD45R/B220-positive gated events with the forward and side light-scatter characteristics of viable bone marrow cells.      Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.
    PE Rat Anti-Mouse CD268 (BAFF-R)
    Multicolor flow cytometric analysis of mouse CD268 (BAFF-R) expression.      Left Panel - Spleen. BALB/c splenic leucocytes were stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553092/561880), and either PE Rat IgG1, κ Isotype Control (Cat. No. 554685; Left Plot), or PE Rat Anti-Mouse CD268 (BAFF-R) antibody (Cat. No. 565783; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD268 (BAFF-R) [or Ig Isotype control staining] versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes.      Right Panel: Bone Marrow. BALB/c bone marrow cells were stained with APC Rat Anti-Mouse CD45R/B220 and BD Horizon™ BUV395 Rat Anti-Mouse IgM (Cat. No. 564025) antibodies, and either PE Rat IgG1, κ Isotype Control (Left Plot) or PE Rat Anti-Mouse CD268 (BAFF-R) antibody (Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD268 (BAFF-R) [or Ig Isotype control staining] versus IgM were derived from CD45R/B220-positive gated events with the forward and side light-scatter characteristics of viable bone marrow cells.      Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.
    Product Details
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    BD Pharmingen™
    Tnfrsf13c; TR13C; BAFF-R; Baffr; Bcmd; Bcmd-1; BLyS receptor 3; Br3; Lvis22
    Mouse (QC Testing)
    Rat WI, also known as Wistar (outbred) IgG1, κ
    Mouse BAFF-R Recombinant Protein
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    72049
    AB_2739353
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    5. An isotype control should be used at the same concentration as the antibody of interest.
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    565783 Rev. 1
    Antibody Details
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    7H22-E16

    The 7H22-E16 monoclonal antibody specifically binds to CD268, which is also known as, B cell-activating factor receptor (BAFF-R), or BAFF Receptor 3 (BR3). CD268 is a type III transmembrane protein that is likewise known as Tumor necrosis factor receptor superfamily member 13C (Tnfrsf13c). CD268/BAFF-R is a receptor for CD257 (also known as, BAFF, Blys, TALL-1, or THANK) and is expressed on B cells and a subset of activated/memory CD4+ T cells. B cells express two other BAFF receptors, CD267/TACI, or CD269/BCMA, at various times during their differentiation. CD268/BAFF-R expression starts at the transitional stage of B cell development and increases throughout B cell maturation. CD267/BAFF-R mediates most BAFF-dependent functions including B cell survival, the activation of B cell proliferation and immunoglobulin secretion, and the costimulation of T cells. Overexpression of BAFF in mice and humans is associated with autoimmunity, whereas CD268/BAFF-R deficiency may cause severe impairment in humoral responses.

    565783 Rev. 1
    Format Details
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    PE
    R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    PE
    Yellow-Green 488 nm, 532 nm, 561 nm
    496 nm, 566 nm
    576 nm
    565783 Rev.1
    Citations & References
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    Development References (3)

    1. Giltiay NV, Lu Y, Allman D, Jørgensen TN, Li X. The adaptor molecule Act1 regulates BAFF responsiveness and self-reactive B cell selection during transitional B cell maturation.. J Immunol. 2010; 185(1):99-109. (Clone-specific: Flow cytometry). View Reference
    2. Naradikian MS, Perate AR, Cancro MP. BAFF receptors and ligands create independent homeostatic niches for B cell subsets.. Curr Opin Immunol. 2015; 34:126-129. (Biology). View Reference
    3. Ng LG, Sutherland AP, Newton R, et al. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells.. J Immunol. 2004; 173(2):807-17. (Immunogen: Flow cytometry). View Reference
    565783 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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