BD Horizon™ BV480 Rat Anti-Mouse F4/80

The T45-2342 monoclonal antibody recognizes the mouse F4/80 antigen which is also known as EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1). F4/80 is a 160 kDa glycoprotein that belongs to the EGF-TM7 family of seven-transmembrane spanning cell surface molecules. It is expressed on the surface of granulocytes and a wide range of mature tissue macrophages including, Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria macrophages, and Langerhans cells. F4/80 expression has also been reported on subpopulations of dendritic cells. F4/80 expression is heterogeneous and may be increased during inflammatory responses as observed in various mouse models of colitis, diabetes and brain injury.

The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye has been exclusively developed by BD Biosciences as an optimal dye for imaging applications. BD Horizon BV480 has an Ex Max at 436 nm and Em Max at 478 nm. The use of a mounting reagent (eg, ProLong® Gold) is highly recommended to maximize the photostability of BV480.

For confocal microscopy systems, a 440 nm laser is the optimal excitation source with optimal emission collection centered at 477 nm. BV480 fluorescence can also be excited well by 405 nm and 458 nm lasers.

For epifluorescence microscopes with broad spectrum excitation sources,  the recommended excitation and emission filters are 445/20 nm and 485/20 nm bandpass filters, respectively.  For specific multicolor imaging applications, the exact filter configurations should be optimized by the end user. For additional instrument/filter configuration information, please visit http://www.bdbiosciences.com/research/cellularimaging.