Flow cytometric analysis of CD46 expression on human peripheral blood lymphocytes. Whole blood was stained with either APC Mouse IgG2a, κ Isotype Control (Cat. No. 550882; dashed line histogram) or APC Mouse Anti-Human CD46 (Cat. No. 564253; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD™ LSR II.
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Flow cytometric analysis of CD46 expression on human peripheral blood lymphocytes. Whole blood was stained with either APC Mouse IgG2a, κ Isotype Control (Cat. No. 550882; dashed line histogram) or APC Mouse Anti-Human CD46 (Cat. No. 564253; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD™ LSR II.
Product Details
BD Pharmingen™
AHUS2; Membrane cofactor protein; MCP; MIC10; TLX; TRA2.10
Human (QC Testing)
Mouse IgG2a, κ
Flow cytometry (Routinely Tested)
5 µl
IV N24
4179
AB_2738705
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Solution 10X Concentrate CE_IVD
Size
100 mL
Cat No.
349202
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
APC Mouse IgG2a κ Isotype Control RUO
Size
0.1 mg
Cat No.
550882
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564253 Rev. 2
Antibody Details
E4.3
The E4.3 monoclonal antibody specifically binds to CD46. CD46 is also known as membrane cofactor protein (MCP). CD46 is a type I membrane glycoprotein composed of two non-disulfide linked α (66 kDa) and β (56 kDa) chains expressed on lymphocytes, monocytes and granulocytes. It is not expressed on erythrocytes or platelets. There are four isoforms of the dimer which function as complement regulatory factors. CD46 promotes the enzymatic degradation of activated C3b and/or C4b deposited on host cells. It also serves as the measles virus receptor.
564253 Rev. 2
Format Details
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
564253 Rev.2
Citations & References
Development References (5)
-
Hu Ly-m5: a unique antigen physically associated with HLA molecules. Hu Ly-m5: a unique antigen physically associated with HLA molecules. Hum Immunol. 1983; 7(1):1-15. (Immunogen). View Reference
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Johnstone RW, Loveland BE, McKenzie IF. Identification and quantification of complement regulator CD46 on normal human tissues. Immunology. 1993; 79(3):341-347. (Immunogen). View Reference
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Kurita M, Yanagi Y, Hara T, Nagasawa S, Matsumoto M, Seya T. Human lymphocytes are more susceptible to measles virus than granulocytes, which is attributable to the phenotypic differences of their membrane cofactor protein (CD46). Immunol Lett. 1995; 48(2):91-95. (Biology). View Reference
-
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
564253 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
