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    BV711 Mouse Anti-Rat CD90/Mouse CD90.1
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    This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [740727] or for additional support, contact your local applications specialist. Contact Us #
    BV711 Mouse Anti-Rat CD90/Mouse CD90.1
    Two-color flow cytometric analysis of CD90 expression on rat splenocytes. Lewis rat splenic leucocytes were stained with BD Horizon™ BV711 Mouse Anti-Rat CD90 (Cat. No. 563772) and FITC Rat Anti-Mouse CD3 (Cat. No. 554832/559975) antibodies. A two-color flow cytometric dot plot showing the correlated expression of CD90 versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.
    BV711 Mouse Anti-Rat CD90/Mouse CD90.1
    Two-color flow cytometric analysis of CD90 expression on rat splenocytes. Lewis rat splenic leucocytes were stained with BD Horizon™ BV711 Mouse Anti-Rat CD90 (Cat. No. 563772) and FITC Rat Anti-Mouse CD3 (Cat. No. 554832/559975) antibodies. A two-color flow cytometric dot plot showing the correlated expression of CD90 versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.
    Product Details
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    BD Horizon™
    Rat Thy-1; Mouse Thy-1.1
    Rat (QC Testing), Mouse (Tested in Development), Rabbit,Guinea Pig (Reported)
    Mouse BALB/c IgG1, κ
    Rat Thymocyte Thy-1 Antigen
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    AB_2738418
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV711 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV711 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    5. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
    6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
    7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    8. BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
    9. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
    10. Cy is a trademark of GE Healthcare.
    11. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    563772 Rev. 2
    Antibody Details
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    OX-7
    563772 Rev. 2
    Format Details
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    BV711
    The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV711
    Violet 405 nm
    407 nm
    713 nm
    563772 Rev.2
    Citations & References
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    Development References (15)

    1. Bañuls MP, Alvarez A, Ferrero I, Zapata A, Ardavin C. Cell-surface marker analysis of rat thymic dendritic cells. Immunology. 1993; 79(2):298-304. (Clone-specific: Flow cytometry). View Reference
    2. Campbell DG, Gagnon J, Reid KB, Williams AF. Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region. Biochem J. 1981; 195(1):15-30. (Clone-specific: Immunoaffinity chromatography). View Reference
    3. Chen-Woan M, Delaney CP, Fournier V, et al. In vitro characterization of rat bone marrow-derived dendritic cells and their precursors. J Leukoc Biol. 1996; 59(2):196-207. (Clone-specific: Cell separation, Functional assay, Immunocytochemistry (cytospins)). View Reference
    4. Crook K and Hunt SV. Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol. 1996; 4:235-246. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
    5. Dráberová L, Amoui M, and Dráber P. Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains. Immunology. 1996; 87(1):141-148. (Clone-specific: Activation, Western blot). View Reference
    6. Garnett D, Barclay AN, Carmo AM, Beyers AD. The association of the protein tyrosine kinases p56lck and p60fyn with the glycosyl phosphatidylinositol-anchored proteins Thy-1 and CD48 in rat thymocytes is dependent on the state of cellular activation. Eur J Immunol. 1993; 23(10):2540-2544. (Clone-specific: Immunoprecipitation). View Reference
    7. Hermans MH, Opstelten D. In situ visualization of hemopoietic cell subsets and stromal elements in rat and mouse bone marrow by immunostaining of frozen sections. J Histochem Cytochem. 1991; 39(12):1627-1634. (Clone-specific: Immunoprecipitation). View Reference
    8. Hosseinzadeh H, Goldschneider I. Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues. J Immunol. 1993; 150(5):1670-1679. (Clone-specific: Flow cytometry). View Reference
    9. Ishizu A, Ishikura H, Nakamaru Y et al. Thy-1 induced on rat endothelium regulates vascular permeability at sites of inflammation. Int Immunol. 1995; 7:1939-1947. (Clone-specific: Immunoprecipitation). View Reference
    10. Kawachi H, Orikasa M, Matsui K, et al. Epitope-specific induction of mesangial lesions with proteinuria by a MoAb against mesangial cell surface antigen. Clin Exp Immunol. 1992; 88(3):399-404. (Clone-specific: Immunofluorescence, Western blot). View Reference
    11. Liu L, Zhang M, Jenkins C, MacPherson GG. Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression. J Immunol. 1998; 161(3):1146-1155. (Clone-specific: Flow cytometry). View Reference
    12. Mason DW, Williams AF. The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem J. 1980; 187(1):1-20. (Immunogen: Flow cytometry, Radioimmunoassay). View Reference
    13. Nakashima I, Zhang YH, Rahman SM, et al. Evidence of synergy between Thy-1 and CD3/TCR complex in signal delivery to murine thymocytes for cell death. J Immunol. 1991; 147(4):1153-1162. (Clone-specific: Activation, Calcium Flux, Functional assay). View Reference
    14. Paul LC, Rennke HG, Milford EL, and Carpenter CB. Thy-1.1 in glomeruli of rat kidneys. Kidney Int. 1984; 25:771-777. (Clone-specific: Electron microscopy, Immunohistochemistry). View Reference
    15. Payer E, Elbe A, Stingl G. Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells. J Immunol. 1991; 146(8):2536-2543. (Clone-specific: Flow cytometry). View Reference
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    563772 Rev. 2

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    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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