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FITC Rat Anti-Mouse CD335 (NKp46)
FITC Rat Anti-Mouse CD335 (NKp46)
Flow cytometric analysis of FITC anti-mouse CD335 (NKp46) expression on mouse splenocytes. C57BL/6 and BALB/c mouse spleen cells were stained separately with FITC anti-mouse CD335 (NKp46) antibody.  After washing, C57BL/6 cells were stained with PE anti-mouse NK-1.1(NKR-P1B and NKR-P1C) antibody (Cat. No.553165; left panel) and BALB/c cells were stained with PE anti-mouse CD49b(DX5) (Cat. No.553858; right panel). Two-color dot plots showing the correlated expression patterns of CD335/NKp46 and either NK1.1/CD161 (C57BL/6 cells; left panel) or DX5/CD49b (BALB/c cells; right panel) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.
Flow cytometric analysis of FITC anti-mouse CD335 (NKp46) expression on mouse splenocytes. C57BL/6 and BALB/c mouse spleen cells were stained separately with FITC anti-mouse CD335 (NKp46) antibody.  After washing, C57BL/6 cells were stained with PE anti-mouse NK-1.1(NKR-P1B and NKR-P1C) antibody (Cat. No.553165; left panel) and BALB/c cells were stained with PE anti-mouse CD49b(DX5) (Cat. No.553858; right panel). Two-color dot plots showing the correlated expression patterns of CD335/NKp46 and either NK1.1/CD161 (C57BL/6 cells; left panel) or DX5/CD49b (BALB/c cells; right panel) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.
Product Details
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BD Pharmingen™
Ncr1; NK-p46; NKp46; mNKp46; MAR1; mAR-1; mouse activating receptor 1; Ly94
Mouse (QC Testing)
Rat IgG2a, κ
Mouse NKP46 Recombinant Protein
Flow cytometry (Routinely Tested)
0.5 mg/ml
17086
AB_1727465
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560756 Rev. 1
Antibody Details
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29A1.4

The monoclonal antibody 29A1.4 specifically binds to mouse CD335, also known as NKp46. NKp46 is a 46 kDa type I transmembrane glycoprotein that is a member of the natural cytotoxicity receptor (NCR) family and immunoglobulin superfamily. NKp46 is encoded by the Ncr1 gene located on chromosome 7. NKp46 functions as a cytotoxicity triggering receptor and is selectively expressed by immature and mature NK cells in all mouse strains tested. NKp46 is detected on a minute fraction of NK-like T cells (less than 2% of NKp46+ express CD3e) but not on CD1d-restricted NKT cells from C57BL/6 mice. When immobilized on tissue culture plates, the 29A1.4 antibody reportedly stimulates NK cells to produce interferon-gamma and to release their cytoplasmic granule contents. Although the ligands for the NKp46 receptor have not been fully characterized, recent evidence indicates that this receptor plays an important role in the NK cell-mediated recognition and killing of some virus-infected cells and tumor cells. The immunogen used to generate the 29A1.4 clone was mouse NKp46-Fc recombinant protein.

560756 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
FITC
Blue 488 nm
494 nm
518 nm
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Citations & References
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Development References (4)

  1. Biassoni R, Pessino A, Bottino C, Pende D, Moretta L, Moretta A. The murine homologue of the human NKp46, a triggering receptor involved in the induction of natural cytotoxicity. Eur J Immunol. 1999; 29(3):1014-1020. (Biology). View Reference
  2. Gazit R, Gruda R, Elboim M, et al. Lethal influenza infection in the absence of the natural killer cell receptor gene Ncr1. Nat Immunol. 2006; 7(5):517-523. (Biology). View Reference
  3. Joncker NT, Fernandez NC, Treiner E, Vivier E, Raulet DH. NK cell responsiveness is tuned commensurate with the number of inhibitory receptors for self-MHC class I: the rheostat model. J Immunol. 2009; 182(8):4572-4580. (Clone-specific: Flow cytometry). View Reference
  4. Walzer T, Blery M, Chaix J, et al. Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46. Proc Natl Acad Sci U S A. 2007; 104(9):3384-3389. (Clone-specific: Activation, Flow cytometry). View Reference
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560756 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.