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    APC Rat anti-Mouse FR4 (Folate receptor 4)
    APC Rat anti-Mouse FR4 (Folate receptor 4)
    Flow cytometric analysis of APC anti-mouse FR4 on mouse splenocytes. Splenocytes from BALB/c mice were stained simultaneously with FITC Rat Anti-Mouse CD25 (clone 7D4, Cat. No. 553072), PE Rat Anti-Mouse CD4 (clone RM4-5, Cat. No. 553048) and APC Rat anti-Mouse FR4.  The left panel shows the gated lymphocyte populations used to generate the histograms in the right panel. The histogram shows expression of FR4 as CD4+CD25+ gated lymphocytes (solid line) and CD25- gated lymphocytes (dashed line). Flow cytometry was performed on a BD FACSCalibur™ System.
    APC Rat anti-Mouse FR4 (Folate receptor 4)
    Flow cytometric analysis of APC anti-mouse FR4 on mouse splenocytes. Splenocytes from BALB/c mice were stained simultaneously with FITC Rat Anti-Mouse CD25 (clone 7D4, Cat. No. 553072), PE Rat Anti-Mouse CD4 (clone RM4-5, Cat. No. 553048) and APC Rat anti-Mouse FR4.  The left panel shows the gated lymphocyte populations used to generate the histograms in the right panel. The histogram shows expression of FR4 as CD4+CD25+ gated lymphocytes (solid line) and CD25- gated lymphocytes (dashed line). Flow cytometry was performed on a BD FACSCalibur™ System.
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    BD Pharmingen™
    Folate receptor 4, FBP, FBP3, FRdelta, FRd
    Mouse (QC Testing)
    Rat WI, also known as Wistar (outbred) IgG1
    Mouse CD4+ CD25+ T cells
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    64931
    AB_1645227
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
    4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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    560318 Rev. 1
    Antibody Details
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    12A5

    The monoclonal antibodies TH6 and 12A5 recognize Folate Receptor 4 (FR4), also known as the membrane folate-binding protein 3 (FBP3). FR4 is a heavily glycosylated 35 kD receptor expressed exclusively in lymphoid tissue and an isoform of the family of receptors that recognize the essential nutrient folic acid. Natural T regulatory cells constitutively express high levels of FR4.  Differential expression of FR4 in combination with CD25 can distinguish four functionally distinct CD4+ T cell subpopulations; Natural Tregs, effector T cells, memory-like T cells and Naïve T cells. FR4hi CD25+ expressing CD4+ T cells also express high amounts of Foxp3, GITR and CTLA-4.

    Monoclonal antibody TH6 and 12A5 stained CD25+CD4+ T cells at a higher level than other CD4+ or CD8+ T cells. In addition, in vivo injection of TH6 monoclonal antibody reduced the number of CD25+CD4+ T cells and CD25-CD4+ T cells in peripheral blood. Clone 12A5 has been demonstrated to work in western blot. Clones TH6 and 12A5 do not block one another in a flow cytometric assay.

    560318 Rev. 1
    Format Details
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    APC
    Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    APC
    Red 627-640 nm
    651 nm
    660 nm
    560318 Rev.1
    Citations & References
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    Development References (3)

    1. Rothberg KG, Ying YS, Kolhouse JF, Kamen BA, Anderson RG. The glycophospholipid-linked folate receptor internalizes folate without entering the clathrin-coated pit endocytic pathway. J Cell Biol. 1990; 110(3):637-649. (Biology). View Reference
    2. Spiegelstein O, Eudy JD, Finnell RH. Identification of two putative novel folate receptor genes in humans and mouse. Gene. 2000; 258(1-2):117-125. (Biology). View Reference
    3. Yamaguchi T, Hirota K, Nagahama K, et al. Control of immune responses by antigen-specific regulatory T cells expressing the folate receptor.. Immunity. 2007; 27(1):145-59. (Biology). View Reference
    560318 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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