PE Mouse Anti-Human CD220

BD Pharmingen™ PE Mouse Anti-Human CD220

Name: PE Mouse Anti-Human CD220
Brand: BD Pharmingen™
SKU: 559955

Clone 3B6/IR

(RUO)

Flow cytometric analysis of CD220 expression on human peripheral blood monocytes. Human whole blood was stained with either PE Mouse Anti-Human CD220 (Cat. No. 559955; solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of intact monocytes.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Insulin Receptor; Insulin R; IR; INSR; HHF5

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

RRID: AB_397392

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Solution 10X Concentrate CE_IVD

Size 100 mL Cat No. 349202

Lysing Buffer RUO

Size 100 mL Cat No. 555899

PE Mouse IgG1, κ Isotype Control RUO

Size 100 Tests Cat No. 555749

559955 Rev. 2

Antibody Details

3B6/IR

The 3B6 monoclonal antibody specifically recognizes the Insulin Receptor (IR), a glycoprotein composed of two extracellular α-subunits (130 kDa) and two transmembrane β-subunits (95 kDa). It is expressed on human hematopoietic and non-hematopoietic cells, but unlike the insulin-like growth factor receptor (IGF-IR), which is ubiquitous, the insulin receptor is restricted to major target tissues of insulin action. Upon binding insulin, the IR subunits form a heterotetramer of two α and two β subunits resulting in autophosphorylation and activation of the tyrosine kinase activity of the receptor. This ligand-receptor interaction is important in regulating cell metabolism and growth. 3B6/IR monoclonal antibody reacts similarly to anti-human IR α, clone 47-9, an α-subunit antibody.

559955 Rev. 2

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

559955 Rev.2

Citations & References

Development References (4)

Brindle NP, Tavare JM, Dickens M, Whittaker J, Siddle K. Anti-(insulin receptor) monoclonal antibody-stimulated tyrosine phosphorylation in cells transfected with human insulin receptor cDNA. Biochem J. 1990; 268(3):615-620. (Biology). View Reference
Prigent SA, Stanley KK, Siddle K. Identification of epitopes on the human insulin receptor reacting with rabbit polyclonal antisera and mouse monoclonal antibodies. J Biol Chem. 1990; 265(17):9970-9979. (Biology). View Reference
Soos MA, O'Brien RM, Brindle NP, et al. Monoclonal antibodies to the insulin receptor mimic metabolic effects of insulin but do not stimulate receptor autophosphorylation in transfected NIH 3T3 fibroblasts.. Proc Natl Acad Sci USA. 1989; 86(14):5217-21. (Biology). View Reference
Soos MA, Siddle K, Baron MD, et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor.. Biochem J. 1986; 235(1):199-208. (Biology). View Reference

View All (4)

559955 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.