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    HRP Rat Anti-Mouse IgG1
    Product Details
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    BD Pharmingen™
    Igh-4; Ighg1; Immunoglobulin heavy constant gamma 1
    Mouse (QC Testing)
    Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
    Mouse IgG1, κ Antibody
    ELISA (Routinely Tested)
    16017
    AB_397292
    Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.
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    Recommended Assay Procedures

    Investigators are encouraged to titrate, with a suggested starting dilution of 1:1000.  For sandwich ELISA HRP Rat Anti-Mouse IgG1 is optimal for detection with Purified Rat Anti-Mouse IgG1 (Cat. No. 553445) for capture.

    Warning:  HRP Rat Anti-Mouse IgG1 contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

    Hazard statements

    May cause an allergic skin reaction.

    Precautionary statements

    Wear protective gloves / eye protection.

    Wear protective clothing.

    Avoid breathing mist/vapours/spray.

    If skin irritation or rash occurs: Get medical advice/attention.

    IF ON SKIN: Wash with plenty of water.

    Dispose of contents/container in accordance with local/regional/national/international regulations.

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    3. ProClin is a trademark of Rohm and Haas Company.
    4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    559626 Rev. 9
    Antibody Details
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    X56

    The X56 monoclonal antibody specifically binds to mouse IgG1 of Igh-C[a] and Igh-C[b] haplotypes. It does not crossreact with other Ig isotypes.

    559626 Rev. 9
    Format Details
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    HRP
    Horseradish Peroxidase is an enzyme that is capable of cleaving a substrate into hydrogen peroxide which can then be detected in solution or in situ. Antibodies, avidins, and other specific binding molecules conjugated with horseradish peroxidase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.
    HRP
    559626 Rev.9
    Citations & References
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    Development References (2)

    1. Hao Z, Duncan GS, Su YW, et al.. The E3 ubiquitin ligase Mule acts through the ATM-p53 axis to maintain B lymphocyte homeostasis. J Exp Med. 2012; 209(1):173-186. (Methodology: ELISA). View Reference
    2. Kita-Furuyama M, Nagayama Y, Pichurin P, McLachlan SM, Rapoport B, Eguchi K. Dendritic cells infected with adenovirus expressing the thyrotrophin receptor induce Graves' hyperthyroidism in BALB/c mice. Clin Exp Immunol. 2003; 131(2):234-240. (Methodology: ELISA). View Reference
    559626 Rev. 9

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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